Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, NCI-H1299 cells, HeLa cells, HepG2 cells, pig brain tissue, rat brain tissue, mouse brain |
| Positive IHC detected in | mouse heart tissue, human colon tissue, mouse skeletal muscle tissue, rat heart tissue, rat skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| FC | See 1 publications below |
Product Information
66735-1-Ig targets Dystroglycan in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Dystroglycan fusion protein Ag27222 相同性解析による交差性が予測される生物種 |
| Full Name | dystroglycan 1 (dystrophin-associated glycoprotein 1) |
| Calculated molecular weight | 97 kDa |
| Observed molecular weight | 43 kDa, 30 kDa |
| GenBank accession number | BC012740 |
| Gene Symbol | Dystroglycan |
| Gene ID (NCBI) | 1605 |
| RRID | AB_2882085 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q14118 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Dystroglycan, also known as DAG1 or DG, was originally isolated from skeletal muscle as an integral membrane component of the dystrophin-glycoprotein complex (DGC). In addition to skeletal muscle, dystroglycan is strongly expressed in heart and smooth muscle, as well as many non-muscle tissues including brain and peripheral nerve (PMID: 12556455). The dystroglycan is involved in a number of processes including laminin and basement membrane assembly, sarcolemmal stability, cell survival, peripheral nerve myelination, nodal structure, cell migration, and epithelial polarization. Dystroglycan consists of two subunits (alpha and beta), which are translated from a single mRNA as a propeptide that is proteolytically cleaved into two noncovalently associated proteins (PMID: 16410545). Alpha-dystroglycan is a 156-kDa extracellular peripheral glycoprotein, while beta-dystroglycan is a 43-kDa transmembrane protein (PMID: 9858474). The 43-kDa beta-dystroglycan can be cleaved into a ~30-kDa form (PMID: 14678802; 18458097; 17255331).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Dystroglycan antibody 66735-1-Ig | Download protocol |
| IHC protocol for Dystroglycan antibody 66735-1-Ig | Download protocol |
| IF protocol for Dystroglycan antibody 66735-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Proc Natl Acad Sci U S A CACNA1S haploinsufficiency confers resistance to New World arenavirus infection. | ||
bioRxiv ER Aggregation Causes Synaptic Protein Imbalance in Retinitis Pigmentosa Mutant Photoreceptor Neurons |