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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Dystroglycan Monoclonal antibody (66735-1-Ig)

    WB analysis using 66735-1-Ig (same clone as 66735-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66735-1-Ig (Dystroglycan antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66735-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using Dystroglycan Monoclonal antibody (66735-1-Ig)

    IHC staining of mouse heart using 66735-1-Ig (same clone as 66735-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66735-1-Ig (Dystroglycan antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66735-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using Dystroglycan Monoclonal antibody (66735-1-Ig)

    IHC staining of mouse heart using 66735-1-Ig (same clone as 66735-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66735-1-Ig (Dystroglycan antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66735-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using Dystroglycan Monoclonal antibody (66735-1-Ig)

    IHC staining of mouse skeletal muscle using 66735-1-Ig (same clone as 66735-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 66735-1-Ig (Dystroglycan antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66735-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using Dystroglycan Monoclonal antibody (66735-1-Ig)

    IHC staining of mouse skeletal muscle using 66735-1-Ig (same clone as 66735-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 66735-1-Ig (Dystroglycan antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66735-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat heart tissue using Dystroglycan Monoclonal antibody (66735-1-Ig)

    IHC staining of rat heart using 66735-1-Ig (same clone as 66735-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 66735-1-Ig (Dystroglycan antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66735-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat heart tissue using Dystroglycan Monoclonal antibody (66735-1-Ig)

    IHC staining of rat heart using 66735-1-Ig (same clone as 66735-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 66735-1-Ig (Dystroglycan antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66735-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat skeletal muscle tissue using Dystroglycan Monoclonal antibody (66735-1-Ig)

    IHC staining of rat skeletal muscle using 66735-1-Ig (same clone as 66735-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 66735-1-Ig (Dystroglycan antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66735-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat skeletal muscle tissue using Dystroglycan Monoclonal antibody (66735-1-Ig)

    IHC staining of rat skeletal muscle using 66735-1-Ig (same clone as 66735-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 66735-1-Ig (Dystroglycan antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66735-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using Dystroglycan Monoclonal antibody (66735-1-Ig)

    IHC staining of human colon using 66735-1-Ig (same clone as 66735-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66735-1-Ig (Dystroglycan antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66735-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Dystroglycan Monoclonal antibody (66735-1-Ig)

    IF Staining of HeLa using 66735-1-Ig (same clone as 66735-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Dystroglycan antibody (66735-1-Ig, Clone: 2B1G12 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66735-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using Dystroglycan Monoclonal antibody (66735-1-Ig)

    IF Staining of A549 using 66735-1-Ig (same clone as 66735-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using Dystroglycan antibody (66735-1-Ig, Clone: 2B1G12 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 66735-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

Dystroglycan Monoclonal antibody, PBS Only

Dystroglycan Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66735-1-PBS
Clone No.2B1G12

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

DAG1, DAG, Beta DG, Alpha DG Beta dystroglycan, AGRNR

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66735-1-PBS targets Dystroglycan in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Dystroglycan fusion protein Ag27222 相同性解析による交差性が予測される生物種
Full Name dystroglycan 1 (dystrophin-associated glycoprotein 1)
Calculated molecular weight 97 kDa
Observed molecular weight 43 kDa, 30 kDa
GenBank accession numberBC012740
Gene Symbol Dystroglycan
Gene ID (NCBI) 1605
RRIDAB_2882085
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ14118
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Dystroglycan, also known as DAG1 or DG, was originally isolated from skeletal muscle as an integral membrane component of the dystrophin-glycoprotein complex (DGC). In addition to skeletal muscle, dystroglycan is strongly expressed in heart and smooth muscle, as well as many non-muscle tissues including brain and peripheral nerve (PMID: 12556455). The dystroglycan is involved in a number of processes including laminin and basement membrane assembly, sarcolemmal stability, cell survival, peripheral nerve myelination, nodal structure, cell migration, and epithelial polarization. Dystroglycan consists of two subunits (alpha and beta), which are translated from a single mRNA as a propeptide that is proteolytically cleaved into two noncovalently associated proteins (PMID: 16410545). Alpha-dystroglycan is a 156-kDa extracellular peripheral glycoprotein, while beta-dystroglycan is a 43-kDa transmembrane protein (PMID: 9858474). The 43-kDa beta-dystroglycan can be cleaved into a ~30-kDa form (PMID: 14678802; 18458097; 17255331).