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Species Reactivity

Species Reactivity

Conjugate

Conjugate

DAPK1 Monoclonal antibody

DAPK1 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1E2F9

Cat no : 67815-1-Ig

Synonyms

DAP kinase 1, DAPK, DAPK1, DKFZp781I035

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DAPK1 Monoclonal antibody (67815-1-Ig)

    WB analysis using 67815-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67815-1-Ig (DAPK1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using DAPK1 Monoclonal antibody (67815-1-Ig)

    IHC staining of human breast cancer using 67815-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67815-1-Ig (DAPK1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using DAPK1 Monoclonal antibody (67815-1-Ig)

    IHC staining of human placenta using 67815-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67815-1-Ig (DAPK1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using DAPK1 Monoclonal antibody (67815-1-Ig)

    IHC staining of human stomach cancer using 67815-1-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67815-1-Ig (DAPK1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skin tissue using DAPK1 Monoclonal antibody (67815-1-Ig)

    IHC staining of mouse skin using 67815-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 67815-1-Ig (DAPK1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using DAPK1 Monoclonal antibody (67815-1-Ig)

    IHC staining of mouse small intestine using 67815-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 67815-1-Ig (DAPK1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat small intestine tissue using DAPK1 Monoclonal antibody (67815-1-Ig)

    IHC staining of rat small intestine using 67815-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 67815-1-Ig (DAPK1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using DAPK1 Monoclonal antibody (67815-1-Ig)

    IHC staining of human breast cancer using 67815-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67815-1-Ig (DAPK1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using DAPK1 Monoclonal antibody (67815-1-Ig)

    IF Staining of human breast cancer using 67815-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using DAPK1 antibody (67815-1-Ig, Clone: 1E2F9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HCT 116 cells using DAPK1 Monoclonal antibody (67815-1-Ig)

    IF Staining of HCT 116 using 67815-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HCT 116 cells using DAPK1 antibody (67815-1-Ig, Clone: 1E2F9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inLNCaP cells, A549 cells, HeLa cells, HepG2 cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, 4T1 cells
Positive IHC detected inhuman breast cancer tissue, human placenta tissue, human stomach cancer tissue, mouse skin tissue, mouse small intestine tissue, rat small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman breast cancer tissue, HCT 116 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

67815-1-Ig targets DAPK1 in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Cited Reactivitymouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen DAPK1 fusion protein Ag29838 相同性解析による交差性が予測される生物種
Full Name death-associated protein kinase 1
Calculated molecular weight 1430 aa, 160 kDa
Observed molecular weight160 kDa
GenBank accession numberBC113660
Gene symbol DAPK1
Gene ID (NCBI) 1612
RRIDAB_2918578
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DAPK1(Death-associated protein kinase 1) is a stress-activated tumor suppressor protein that plays a role in both proapoptotic or antiapoptotic signal transduction pathways. Loss of DAPK1 expression is associated with a selective advantage fortumor cells to resist apoptotic stimuli, allowing them to separate from the original tumor; from this point of view, DAPK1 could be considered a tumor metastases inhibitor gene(PMID:17319784).

Protocols

Product Specific Protocols
WB protocol for DAPK1 antibody 67815-1-IgDownload protocol
IHC protocol for DAPK1 antibody 67815-1-IgDownload protocol
IF protocol for DAPK1 antibody 67815-1-IgDownload protocol
FC protocol for DAPK1 antibody 67815-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Cell Commun Signal

P38-DAPK1 axis regulated LC3-associated phagocytosis (LAP) of microglia in an in vitro subarachnoid hemorrhage model

Authors - Xiang-Xin Chen
View Article