DAPK1 Monoclonal antibody

DAPK1 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1E2F9

Cat no : 67815-1-Ig

Synonyms

EC:2.7.11.1, Death-associated protein kinase 1, DAPK, DAP kinase 1, 1E2F9


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Tested Applications

Positive WB detected inLNCaP cells, A549 cells, HeLa cells, HepG2 cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, 4T1 cells
Positive IHC detected inhuman breast cancer tissue, human placenta tissue, human stomach cancer tissue, mouse skin tissue, mouse small intestine tissue, rat small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman breast cancer tissue
Positive IF/ICC detected inHCT 116 cells, HT-1376 cells
Positive FC (Intra) detected inHCT 116 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67815-1-Ig targets DAPK1 in WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen DAPK1 fusion protein Ag29838 相同性解析による交差性が予測される生物種
Full Name death-associated protein kinase 1
Calculated molecular weight 1430 aa, 160 kDa
Observed molecular weight160 kDa
GenBank accession numberBC113660
Gene symbol DAPK1
Gene ID (NCBI) 1612
RRIDAB_2918578
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DAPK1(Death-associated protein kinase 1) is a stress-activated tumor suppressor protein that plays a role in both proapoptotic or antiapoptotic signal transduction pathways. Loss of DAPK1 expression is associated with a selective advantage fortumor cells to resist apoptotic stimuli, allowing them to separate from the original tumor; from this point of view, DAPK1 could be considered a tumor metastases inhibitor gene(PMID:17319784).

Protocols

Product Specific Protocols
WB protocol for DAPK1 antibody 67815-1-IgDownload protocol
IHC protocol for DAPK1 antibody 67815-1-IgDownload protocol
IF protocol for DAPK1 antibody 67815-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Theranostics

Macrophage membrane‒biomimetic nanoparticles target inflammatory microenvironment for epilepsy treatment

Authors - Chao Geng
mouseIF

Cell Commun Signal

P38-DAPK1 axis regulated LC3-associated phagocytosis (LAP) of microglia in an in vitro subarachnoid hemorrhage model

Authors - Xiang-Xin Chen
mouseWB

Cell Death Differ

The PM20D1-NADA pathway protects against Parkinson's disease

Authors - Yunying Yang