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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DAPK1 Monoclonal antibody, PBS Only

DAPK1 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E2F9

Cat no : 67815-1-PBS

Synonyms

EC:2.7.11.1, Death-associated protein kinase 1, DAPK, DAP kinase 1, 1E2F9

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DAPK1 Monoclonal antibody (67815-1-Ig)

    WB analysis using 67815-1-Ig (same clone as 67815-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67815-1-Ig (DAPK1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 67815-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using DAPK1 Monoclonal antibody (67815-1-Ig)

    IHC staining of human breast cancer using 67815-1-Ig (same clone as 67815-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67815-1-Ig (DAPK1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67815-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using DAPK1 Monoclonal antibody (67815-1-Ig)

    IHC staining of human placenta using 67815-1-Ig (same clone as 67815-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67815-1-Ig (DAPK1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67815-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using DAPK1 Monoclonal antibody (67815-1-Ig)

    IHC staining of human stomach cancer using 67815-1-Ig (same clone as 67815-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67815-1-Ig (DAPK1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67815-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skin tissue using DAPK1 Monoclonal antibody (67815-1-Ig)

    IHC staining of mouse skin using 67815-1-Ig (same clone as 67815-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 67815-1-Ig (DAPK1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67815-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using DAPK1 Monoclonal antibody (67815-1-Ig)

    IHC staining of mouse small intestine using 67815-1-Ig (same clone as 67815-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 67815-1-Ig (DAPK1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67815-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat small intestine tissue using DAPK1 Monoclonal antibody (67815-1-Ig)

    IHC staining of rat small intestine using 67815-1-Ig (same clone as 67815-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 67815-1-Ig (DAPK1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67815-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using DAPK1 Monoclonal antibody (67815-1-Ig)

    IHC staining of human breast cancer using 67815-1-Ig (same clone as 67815-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67815-1-Ig (DAPK1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67815-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using DAPK1 Monoclonal antibody (67815-1-Ig)

    IF Staining of human breast cancer using 67815-1-Ig (same clone as 67815-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using DAPK1 antibody (67815-1-Ig, Clone: 1E2F9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67815-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HT-1376 cells using DAPK1 Monoclonal antibody (67815-1-Ig)

    IF Staining of HT-1376 using 67815-1-Ig (same clone as 67815-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HT-1376 cells using DAPK1 antibody (67815-1-Ig, Clone: 1E2F9 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 67815-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HCT 116 cells using DAPK1 Monoclonal antibody (67815-1-Ig)

    IF Staining of HCT 116 using 67815-1-Ig (same clone as 67815-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HCT 116 cells using DAPK1 antibody (67815-1-Ig, Clone: 1E2F9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67815-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HCT 116 cells using DAPK1 Monoclonal antibody (67815-1-Ig)

    FC experiment of HCT 116 using 67815-1-Ig (same clone as 67815-1-PBS)

    1X10^6 HCT 116 cells were intracellularly stained with 0.4 ug Anti-Human DAPK1 (67815-1-Ig, Clone:1E2F9) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67815-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67815-1-PBS targets DAPK1 in WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen DAPK1 fusion protein Ag29838 相同性解析による交差性が予測される生物種
Full Name death-associated protein kinase 1
Calculated molecular weight 1430 aa, 160 kDa
Observed molecular weight160 kDa
GenBank accession numberBC113660
Gene symbol DAPK1
Gene ID (NCBI) 1612
RRIDAB_2918578
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

DAPK1(Death-associated protein kinase 1) is a stress-activated tumor suppressor protein that plays a role in both proapoptotic or antiapoptotic signal transduction pathways. Loss of DAPK1 expression is associated with a selective advantage fortumor cells to resist apoptotic stimuli, allowing them to separate from the original tumor; from this point of view, DAPK1 could be considered a tumor metastases inhibitor gene(PMID:17319784).