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Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using DBR1 Polyclonal antibody (16019-1-AP)

    WB analysis of human brain using 16019-1-AP (same clone as 16019-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 16019-1-AP (DBR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16019-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using DBR1 Polyclonal antibody (16019-1-AP)

    WB analysis of HeLa using 16019-1-AP (same clone as 16019-1-PBS)

    WB result of DBR1 antibody (16019-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DBR1 transfected HeLa cells. This data was developed using the same antibody clone with 16019-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using DBR1 Polyclonal antibody (16019-1-AP)

    WB analysis of HepG2 using 16019-1-AP (same clone as 16019-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16019-1-AP (DBR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16019-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using DBR1 Polyclonal antibody (16019-1-AP)

    IP experiment of HeLa using 16019-1-AP (same clone as 16019-1-PBS)

    IP result of anti-DBR1 (IP:16019-1-AP, 4ug; Detection:16019-1-AP 1:800) with HeLa cells lysate 1560 ug. This data was developed using the same antibody clone with 16019-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using DBR1 Polyclonal antibody (16019-1-AP)

    IP experiment of mouse brain using 16019-1-AP (same clone as 16019-1-PBS)

    IP result of anti-DBR1 (IP:16019-1-AP, 4ug; Detection:16019-1-AP 1:500) with mouse brain tissue lysate 1280 ug. This data was developed using the same antibody clone with 16019-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using DBR1 Polyclonal antibody (16019-1-AP)

    IHC staining of human placenta using 16019-1-AP (same clone as 16019-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta using 16019-1-AP (DBR1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 16019-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using DBR1 Polyclonal antibody (16019-1-AP)

    IHC staining of human placenta using 16019-1-AP (same clone as 16019-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta using 16019-1-AP (DBR1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 16019-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using DBR1 Polyclonal antibody (16019-1-AP)

    IF Staining of HeLa using 16019-1-AP (same clone as 16019-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DBR1 antibody (16019-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 16019-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

DBR1 Polyclonal antibody, PBS Only

DBR1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 16019-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16019-1-PBS targets DBR1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DBR1 fusion protein Ag8830 相同性解析による交差性が予測される生物種
Full Name debranching enzyme homolog 1 (S. cerevisiae)
Calculated molecular weight 544 aa, 62 kDa
Observed molecular weight 70-80 kDa
GenBank accession numberBC009472
Gene Symbol DBR1
Gene ID (NCBI) 51163
RRIDAB_2230316
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UK59
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

DBR1(Lariat debranching enzyme) hydrolyzes 2-prime-to-5-prime branched phosphodiester bonds at the branch point of excised lariat intron RNA and converts them into linear molecules. DBR1 belongs to the lariat debranching enzyme family. Inhibiton of Dbr1 can suppress TDP-43 toxicity in primary neurons, suggesting that Dbr1 could be a potential therapeutic target for ALS and related TDP-43 proteinopathies (PMID: 23104007). This protein has 2 isoforms produced by alternative splicing.