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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using DBR1 Monoclonal antibody (66951-1-Ig)

    WB analysis of HeLa using 66951-1-Ig

    WB result of DBR1 antibody (66951-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DBR1 transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using DBR1 Monoclonal antibody (66951-1-Ig)

    WB analysis using 66951-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66951-1-Ig (DBR1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using DBR1 Monoclonal antibody (66951-1-Ig)

    WB analysis of HeLa using 66951-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66951-1-Ig (DBR1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using DBR1 Monoclonal antibody (66951-1-Ig)

    WB analysis of MCF-7 using 66951-1-Ig

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 66951-1-Ig (DBR1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using DBR1 Monoclonal antibody (66951-1-Ig)

    WB analysis using 66951-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66951-1-Ig (DBR1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using DBR1 Monoclonal antibody (66951-1-Ig)

    WB analysis using 66951-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66951-1-Ig (DBR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using DBR1 Monoclonal antibody (66951-1-Ig)

    WB analysis using 66951-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66951-1-Ig (DBR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using DBR1 Monoclonal antibody (66951-1-Ig)

    IP experiment of HeLa using 66951-1-Ig

    IP result of anti-DBR1 (IP:66951-1-Ig, 4ug; Detection:66951-1-Ig 1:2000) with HeLa cells lysate 1560 ug.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using DBR1 Monoclonal antibody (66951-1-Ig)

    IP experiment of mouse brain using 66951-1-Ig

    IP result of anti-DBR1 (IP:66951-1-Ig, 4ug; Detection:66951-1-Ig 1:2000) with mouse brain tissue lysate 1280 ug.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using DBR1 Monoclonal antibody (66951-1-Ig)

    IHC staining of mouse brain using 66951-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66951-1-Ig (DBR1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using DBR1 Monoclonal antibody (66951-1-Ig)

    IHC staining of mouse brain using 66951-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66951-1-Ig (DBR1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using DBR1 Monoclonal antibody (66951-1-Ig)

    IF Staining of A431 using 66951-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using DBR1 antibody (66951-1-Ig, Clone: 3B5A7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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  • Featured Product
  • KD/KO Validated

DBR1 Monoclonal antibody

DBR1 Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 66951-1-Ig
Clone No.3B5A7

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

DBR1, Lariat debranching enzyme

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman brain tissue, HeLa cells, MCF-7 cells, fetal human brain tissue, pig brain tissue, rat brain tissue, mouse brain tissue, HepG2 cells
Positive IP detected inHeLa cells, mouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66951-1-Ig targets DBR1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Reactivity Human, Mouse, Rat, Pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen DBR1 fusion protein Ag8830 相同性解析による交差性が予測される生物種
Full Name debranching enzyme homolog 1 (S. cerevisiae)
Calculated molecular weight 544 aa, 62 kDa
Observed molecular weight 70-80 kDa
GenBank accession numberBC009472
Gene Symbol DBR1
Gene ID (NCBI) 51163
RRIDAB_2882274
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UK59
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DBR1(Lariat debranching enzyme) hydrolyzes 2-prime-to-5-prime branched phosphodiester bonds at the branch point of excised lariat intron RNA and converts them into linear molecules. DBR1 belongs to the lariat debranching enzyme family. Inhibiton of Dbr1 can suppress TDP-43 toxicity in primary neurons, suggesting that Dbr1 could be a potential therapeutic target for ALS and related TDP-43 proteinopathies (PMID: 23104007). This protein has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for DBR1 antibody 66951-1-IgDownload protocol
IHC protocol for DBR1 antibody 66951-1-IgDownload protocol
IF protocol for DBR1 antibody 66951-1-IgDownload protocol
IP protocol for DBR1 antibody 66951-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols