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  • Featured Product
  • KD/KO Validated

DBR1 Monoclonal antibody, PBS Only

DBR1 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3B5A7

Cat no : 66951-1-PBS

Synonyms

DBR1, Lariat debranching enzyme

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using DBR1 Monoclonal antibody (66951-1-Ig)

    WB analysis of HeLa using 66951-1-Ig (same clone as 66951-1-PBS)

    WB result of DBR1 antibody (66951-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DBR1 transfected HeLa cells. This data was developed using the same antibody clone with 66951-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using DBR1 Monoclonal antibody (66951-1-Ig)

    WB analysis using 66951-1-Ig (same clone as 66951-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66951-1-Ig (DBR1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66951-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using DBR1 Monoclonal antibody (66951-1-Ig)

    WB analysis of HeLa using 66951-1-Ig (same clone as 66951-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66951-1-Ig (DBR1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66951-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using DBR1 Monoclonal antibody (66951-1-Ig)

    WB analysis of MCF-7 using 66951-1-Ig (same clone as 66951-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 66951-1-Ig (DBR1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66951-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using DBR1 Monoclonal antibody (66951-1-Ig)

    WB analysis using 66951-1-Ig (same clone as 66951-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66951-1-Ig (DBR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66951-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using DBR1 Monoclonal antibody (66951-1-Ig)

    WB analysis using 66951-1-Ig (same clone as 66951-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66951-1-Ig (DBR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66951-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using DBR1 Monoclonal antibody (66951-1-Ig)

    WB analysis using 66951-1-Ig (same clone as 66951-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66951-1-Ig (DBR1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66951-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using DBR1 Monoclonal antibody (66951-1-Ig)

    IP experiment of HeLa using 66951-1-Ig (same clone as 66951-1-PBS)

    IP result of anti-DBR1 (IP:66951-1-Ig, 4ug; Detection:66951-1-Ig 1:2000) with HeLa cells lysate 1560 ug. This data was developed using the same antibody clone with 66951-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using DBR1 Monoclonal antibody (66951-1-Ig)

    IP experiment of mouse brain using 66951-1-Ig (same clone as 66951-1-PBS)

    IP result of anti-DBR1 (IP:66951-1-Ig, 4ug; Detection:66951-1-Ig 1:2000) with mouse brain tissue lysate 1280 ug. This data was developed using the same antibody clone with 66951-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using DBR1 Monoclonal antibody (66951-1-Ig)

    IHC staining of mouse brain using 66951-1-Ig (same clone as 66951-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66951-1-Ig (DBR1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66951-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using DBR1 Monoclonal antibody (66951-1-Ig)

    IHC staining of mouse brain using 66951-1-Ig (same clone as 66951-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66951-1-Ig (DBR1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66951-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using DBR1 Monoclonal antibody (66951-1-Ig)

    IF Staining of A431 using 66951-1-Ig (same clone as 66951-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using DBR1 antibody (66951-1-Ig, Clone: 3B5A7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66951-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66951-1-PBS targets DBR1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Reactivity Human, Mouse, Rat, Pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen DBR1 fusion protein Ag8830 相同性解析による交差性が予測される生物種
Full Name debranching enzyme homolog 1 (S. cerevisiae)
Calculated molecular weight 544 aa, 62 kDa
Observed molecular weight 70-80 kDa
GenBank accession numberBC009472
Gene symbol DBR1
Gene ID (NCBI) 51163
RRIDAB_2882274
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

DBR1(Lariat debranching enzyme) hydrolyzes 2-prime-to-5-prime branched phosphodiester bonds at the branch point of excised lariat intron RNA and converts them into linear molecules. DBR1 belongs to the lariat debranching enzyme family. Inhibiton of Dbr1 can suppress TDP-43 toxicity in primary neurons, suggesting that Dbr1 could be a potential therapeutic target for ALS and related TDP-43 proteinopathies (PMID: 23104007). This protein has 2 isoforms produced by alternative splicing.