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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DCBLD2 Polyclonal antibody (30245-1-AP)

    WB analysis using 30245-1-AP (same clone as 30245-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30245-1-AP (DCBLD2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30245-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using DCBLD2 Polyclonal antibody (30245-1-AP)

    IHC staining of human stomach cancer using 30245-1-AP (same clone as 30245-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 30245-1-AP (DCBLD2 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30245-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using DCBLD2 Polyclonal antibody (30245-1-AP)

    IHC staining of human stomach cancer using 30245-1-AP (same clone as 30245-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 30245-1-AP (DCBLD2 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30245-1-PBS in a different storage buffer formulation.

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DCBLD2 Polyclonal antibody, PBS Only

DCBLD2 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 30245-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

30245-1-PBS targets DCBLD2 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DCBLD2 fusion protein Ag32823 相同性解析による交差性が予測される生物種
Full Name discoidin, CUB and LCCL domain containing 2
Calculated molecular weight 775 aa, 89 kDa
Observed molecular weight120 kDa
GenBank accession numberBC029658
Gene Symbol DCBLD2
Gene ID (NCBI) 131566
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96PD2
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

DCBLD2, also known as ESDN or CLCP1, was first identified from human coronary arterial cells by a signal sequence trap method. DCBLD2 regulates proliferation, migration, and invasion in various tumors, including glioma, hypopharyngeal squamous cell carcinoma, and myxofibrosarcoma. The human DCBLD2 protein has a predicted molecular mass of ∼80 kDa based on its amino acid sequence, although it regularly displays an effective molecular mass of ∼130 kDa via SDS-PAGE (PMID: 30902898).