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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DCTD Monoclonal antibody

DCTD Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3D2G12

Cat no : 68357-1-Ig

Synonyms

dCMP deaminase, DCTD, Deoxycytidylate deaminase

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DCTD Monoclonal antibody (68357-1-Ig)

    WB analysis using 68357-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68357-1-Ig (DCTD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using DCTD Monoclonal antibody (68357-1-Ig)

    IF Staining of HepG2 using 68357-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DCTD antibody (68357-1-Ig, Clone: 3D2G12 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inA549 cells, U2OS cells, HeLa cells, LNCaP cells, HEK-293 cells, Jurkat cells, K-562 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

68357-1-Ig targets DCTD in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen DCTD fusion protein Ag10245 相同性解析による交差性が予測される生物種
Full Name dCMP deaminase
Calculated molecular weight 178 aa, 20 kDa
Observed molecular weight18-20 kDa
GenBank accession numberBC001286
Gene symbol DCTD
Gene ID (NCBI) 1635
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DCTD(Deoxycytidylate deaminase) is also named as dCMP deaminase and belongs to the cytidine and deoxycytidylate deaminase family. It catalyzes the deamination of dCMP to dUMP, thus providing the nucleotide substrate for thymidylate synthase. Control of deaminase activity at this juncture in deoxyribonucleotide metabolism is determined by the ratio of dCTP to dTTP in the cell, since the enzyme is allosterically activated by dCTP and inhibited by dTTP(PMID:7685356). It has 2 isoforms produced by alternative splicing and can exsit as a dimer(pMID:8798492).

Protocols

Product Specific Protocols
WB protocol for DCTD antibody 68357-1-IgDownload protocol
IF protocol for DCTD antibody 68357-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Molecules

Teadenol B as a Component of Microorganism-Fermented Tea Extract Inhibited Breast Cancers by Promoting Autophagy

Authors - Ying Zhao
View Article