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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DCTD Monoclonal antibody (68357-1-Ig)

    WB analysis using 68357-1-Ig (same clone as 68357-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68357-1-Ig (DCTD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68357-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using DCTD Monoclonal antibody (68357-1-Ig)

    IF Staining of HepG2 using 68357-1-Ig (same clone as 68357-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DCTD antibody (68357-1-Ig, Clone: 3D2G12 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68357-1-PBS in a different storage buffer formulation.

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DCTD Monoclonal antibody, PBS Only

DCTD Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 68357-1-PBS
Clone No.3D2G12

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

dCMP deaminase, DCTD, Deoxycytidylate deaminase

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68357-1-PBS targets DCTD in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen DCTD fusion protein Ag10245 相同性解析による交差性が予測される生物種
Full Name dCMP deaminase
Calculated molecular weight 178 aa, 20 kDa
Observed molecular weight18-20 kDa
GenBank accession numberBC001286
Gene Symbol DCTD
Gene ID (NCBI) 1635
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP32321
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

DCTD(Deoxycytidylate deaminase) is also named as dCMP deaminase and belongs to the cytidine and deoxycytidylate deaminase family. It catalyzes the deamination of dCMP to dUMP, thus providing the nucleotide substrate for thymidylate synthase. Control of deaminase activity at this juncture in deoxyribonucleotide metabolism is determined by the ratio of dCTP to dTTP in the cell, since the enzyme is allosterically activated by dCTP and inhibited by dTTP(PMID:7685356). It has 2 isoforms produced by alternative splicing and can exsit as a dimer(pMID:8798492).