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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated DCX Polyclonal antibody

DCX Polyclonal Antibody for IF-P

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-13925

Synonyms

Lis-X, Lissencephalin-X, Doublin, doublecortin

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CoraLite® Plus 488-conjugated DCX Polyclonal antib (CL488-13925)

    IF Staining of mouse brain using CL488-13925

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using CoraLite® Plus 488 DCX antibody (CL488-13925) at dilution of 1:50. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive IF-P detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-13925 targets DCX in IF-P applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DCX fusion protein Ag4945 相同性解析による交差性が予測される生物種
Full Name doublecortin
Calculated molecular weight 402 aa, 45 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC027925
Gene symbol DCX
Gene ID (NCBI) 1641
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DCX (Doublecortin) is a microtubule-associated protein that is essential for normal brain development. DCX is expressed in migrating neurons throughout the central and peripheral nervous system during embryonic and postnatal development. Anti-DCX antibodies are widely used for detection of neurogenesis. It's also a common neuron marker since its expression is specific to neurons.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 DCX antibody CL488-13925Download protocol
Standard Protocols
Click here to view our Standard Protocols