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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using CoraLite® Plus 488-conjugated DDHD2 Polyclonal ant (CL488-25203)

    IF Staining of HEK-293 using CL488-25203

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CoraLite® Plus 488 DDHD2 antibody (CL488-25203) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HEK-293 cells using CoraLite® Plus 488-conjugated DDHD2 Polyclonal ant (CL488-25203)

    FC experiment of HEK-293 using CL488-25203

    1x10^6 HEK-293 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Ddhd2 Polyclonal Antibody (CL488-25203)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated DDHD2 Polyclonal antibody

DDHD2 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-25203

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Synonyms

SAM, WWE and DDHD domain-containing protein 1, Phospholipase DDHD2, KIAA0725p, KIAA0725, EC:3.1.1.-

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHEK-293 cells
Positive FC (Intra) detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-25203 targets DDHD2 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DDHD2 fusion protein Ag18373 相同性解析による交差性が予測される生物種
Full Name DDHD domain containing 2
Calculated molecular weight 711 aa, 81 kDa
Observed molecular weight 80-90 kDa
GenBank accession numberBC010504
Gene Symbol DDHD2
Gene ID (NCBI) 23259
RRIDAB_3672788
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO94830
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DDHD2, also known as KIAA0725p, is a member of the intracellular phospholipase A1 (PLA1) protein family which comprises a group of enzymes that hydrolyze the sn-1 ester bond of phospholipids, producing 2-acyl-lysophospholipids and fatty acids. DDHD2 prefers phosphatidic acid as substrate and has a role in efficient membrane trafficking from the Golgi apparatus to the plasma membrane. The gene of DDHD2 maps to chromosome 8p11.23, and encodes a 711-amino-acid protein with a calculated molecular mass of 81 kDa. It has been reported that DDHD2 immunoblot analysis of various tissue extracts revealed two bands of 90 and 85 kDa (PMID: 11788596).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 DDHD2 antibody CL488-25203Download protocol
FC protocol for CL Plus 488 DDHD2 antibody CL488-25203Download protocol
Standard Protocols
Click here to view our Standard Protocols