Validation Data Gallery
Tested Applications
| Positive FC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is N/A per 10^6 cells in a 100 µl suspension or N/A per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL647-98200 targets DDR1/CD167a in FC applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | Recombinant protein 相同性解析による交差性が予測される生物種 |
| Full Name | discoidin domain receptor tyrosine kinase 1 |
| Calculated molecular weight | 101kDa |
| GenBank accession number | NM_001297654.2 |
| Gene Symbol | DDR1 |
| Gene ID (NCBI) | 780 |
| Conjugate | CoraLite® Plus 647 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 654 nm / 674 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q08345-1 |
| Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
DDR1, or CD167a, is a member of the receptor tyrosine kinase (RTK) family, which plays a significant role in various cellular processes including cell proliferation, adhesion, migration, and extracellular matrix (ECM) remodeling. DDR1 is known for its unique discoidin domain that allows it to bind specifically to certain collagens, initiating downstream signaling pathways that can lead to cell transformation and tumor progression. Abnormal activation of DDR1 is closely associated with the development of various solid tumors, and it has been shown that DDR1 can prevent immune cells from infiltrating triple-negative breast cancer (TNBC) and eliminate tumor cells.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 647 DDR1/CD167a antibody CL647-98200 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |