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DDX1 Monoclonal antibody

DDX1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1G10G4

Cat no : 67991-1-Ig

Synonyms

DBP RB, DDX1, DEAD box protein 1, UKVH5d

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DDX1 Monoclonal antibody (67991-1-Ig)

    WB analysis using 67991-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67991-1-Ig (DDX1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of 4T1 cells using DDX1 Monoclonal antibody (67991-1-Ig)

    WB analysis of 4T1 using 67991-1-Ig

    4T1 cells were subjected to SDS PAGE followed by western blot with 67991-1-Ig (DDX1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using DDX1 Monoclonal antibody (67991-1-Ig)

    WB analysis of HeLa using 67991-1-Ig

    WB result of DDX1 antibody (67991-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DDX1 transfected HeLa cells.

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Tested Applications

Positive WB detected inA431 cells, 4T1 cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67991-1-Ig targets DDX1 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen DDX1 fusion protein Ag16774 相同性解析による交差性が予測される生物種
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 1
Calculated molecular weight 740 aa, 82 kDa
Observed molecular weight82 kDa
GenBank accession numberBC012132
Gene symbol DDX1
Gene ID (NCBI) 1653
RRIDAB_2918740
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DDX1 is a DEAD box protein, which is putative RNA helicases with a characteristic asp-glu-ala-asp (DEAD) box motif. DEAD box proteins involve in translation initiation, splicing, and ribosome and spliceosome assembly by altering RNA secondary structure. As a RNA helicase, DDX1 has a role in RNA clearance at DNA double-strand breaks (DSBs), thereby facilitating the template-guided repair of transcriptionally active regions of the genome.

Protocols

Product Specific Protocols
WB protocol for DDX1 antibody 67991-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols