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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated DDX1 Monoclonal antibody

DDX1 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1G10G4

Cat no : CL488-67991

Synonyms

DEAD box protein retinoblastoma, DEAD box protein 1, DBP-RB, DBP RB, ATP-dependent RNA helicase DDX1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CoraLite® Plus 488-conjugated DDX1 Monoclonal anti (CL488-67991)

    IF Staining of A431 using CL488-67991

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite® Plus 488 DDX1 antibody (CL488-67991, Clone: 1G10G4 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated DDX1 Monoclonal anti (CL488-67991)

    FC experiment of HeLa using CL488-67991

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human DDX1 (CL488-67991, Clone:1G10G4) (red), or 0.4 ug x. Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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Tested Applications

Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67991 targets DDX1 in IF/ICC, FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen DDX1 fusion protein Ag16774 相同性解析による交差性が予測される生物種
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 1
Calculated molecular weight 740 aa, 82 kDa
Observed molecular weight82 kDa
GenBank accession numberBC012132
Gene symbol DDX1
Gene ID (NCBI) 1653
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DDX1 is a DEAD box protein, which is putative RNA helicases with a characteristic asp-glu-ala-asp (DEAD) box motif. DEAD box proteins involve in translation initiation, splicing, and ribosome and spliceosome assembly by altering RNA secondary structure. As a RNA helicase, DDX1 has a role in RNA clearance at DNA double-strand breaks (DSBs), thereby facilitating the template-guided repair of transcriptionally active regions of the genome.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 DDX1 antibody CL488-67991Download protocol
Standard Protocols
Click here to view our Standard Protocols