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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DDX46 Recombinant antibody (85065-3-RR)

    WB analysis using 85065-3-RR (same clone as 85065-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85065-3-RR (DDX46 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85065-3-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01807-1

    Sandwich ELISA standard curve of MP01807-1

    Sandwich ELISA standard curve of MP01807-1, Human DDX46 Recombinant Matched Antibody Pair - PBS only. 85065-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag10342. 85065-1-PBS was HRP conjugated as the detection antibody. Range: 0.391-25 ng/mL.

  • Sample test of MP01807-1

    Sample test of MP01807-1

    The mean DDX46 concentration was determined to be 109.98 ng/mL in HEK-293 cell extract based on a 2.70 mg/mL extract load and 35.02 ng/mL in HeLa cell extract based on a 1.50 mg/mL extract load.

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using DDX46 Recombinant antibody (85065-3-RR)

    IF Staining of U-251 using 85065-3-RR (same clone as 85065-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using DDX46 antibody (85065-3-RR, Clone: 242715E10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85065-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01807-2

    Cytometric bead array standard curve of MP01807-2

    Cytometric bead array standard curve of MP01807-2, DDX46 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85065-3-PBS. Detection antibody: 85065-2-PBS. Standard: Ag10342. Range: 0.781-100 ng/mL.

  • Cytometric bead array standard curve of MP01807-1

    Cytometric bead array standard curve of MP01807-1

    Cytometric bead array standard curve of MP01807-1, DDX46 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85065-3-PBS. Detection antibody: 85065-1-PBS. Standard: Ag10342. Range: 0.781-100 ng/mL.

  • Affinity and Kinetic Characterization of 85065-3-RR

    Affinity and Kinetic Characterization of 85065-3-RR

    Biolayer interferometry (BLl) kinetic assays of 85065-3-RR against Human DDX46 were performed. The affinity constant is 51.9 pM.

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DDX46 Recombinant antibody, PBS Only (Capture)

DDX46 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 85065-3-PBS
Clone No.242715E10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

Probable ATP-dependent RNA helicase DDX46, KIAA0801, EC:3.6.4.13, DEAD box protein 46, 242715E10

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85065-3-PBS targets DDX46 as part of a matched antibody pair:

MP01807-1: 85065-3-PBS capture and 85065-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

MP01807-2: 85065-3-PBS capture and 85065-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen DDX46 fusion protein Ag10342 相同性解析による交差性が予測される生物種
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 46
Calculated molecular weight 1031 aa, 117 kDa
Observed molecular weight140 kDa
GenBank accession numberBC012304
Gene Symbol DDX46
Gene ID (NCBI) 9879
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ7L014
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

DDX46, also named as KIAA0801, is a 1031 amino acid protein, which belongs to the DEAD box helicase family. DDX46 has been shown to be required at the early step of pre-spliceosome assembly. It uses the energy released by the hydrolysis of ATP to rearrange local RNA-RNA or protein-RNA interactions. The nuclear DDX46 acted as a negative regulator of the antiviral innate response by recruiting the m6A'eraser' ALKBH5 to induce retention of antiviral innate mRNA in the nucleus.