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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DDX46 Recombinant antibody (85065-3-RR)

    WB analysis using 85065-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85065-3-RR (DDX46 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using DDX46 Recombinant antibody (85065-3-RR)

    IF Staining of U-251 using 85065-3-RR

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using DDX46 antibody (85065-3-RR, Clone: 242715E10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 85065-3-RR

    Affinity and Kinetic Characterization of 85065-3-RR

    Biolayer interferometry (BLl) kinetic assays of 85065-3-RR against Human DDX46 were performed. The affinity constant is 51.9 pM.

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DDX46 Recombinant antibody

DDX46 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 85065-3-RR
Clone No.242715E10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

Probable ATP-dependent RNA helicase DDX46, KIAA0801, EC:3.6.4.13, DEAD box protein 46, 242715E10

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, A549 cells, U-251 cells, NIH/3T3 cells, rat testis tissue
Positive IF/ICC detected inU-251 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85065-3-RR targets DDX46 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen DDX46 fusion protein Ag10342 相同性解析による交差性が予測される生物種
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 46
Calculated molecular weight 1031 aa, 117 kDa
Observed molecular weight140 kDa
GenBank accession numberBC012304
Gene Symbol DDX46
Gene ID (NCBI) 9879
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ7L014
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DDX46, also named as KIAA0801, is a 1031 amino acid protein, which belongs to the DEAD box helicase family. DDX46 has been shown to be required at the early step of pre-spliceosome assembly. It uses the energy released by the hydrolysis of ATP to rearrange local RNA-RNA or protein-RNA interactions. The nuclear DDX46 acted as a negative regulator of the antiviral innate response by recruiting the m6A'eraser' ALKBH5 to induce retention of antiviral innate mRNA in the nucleus.

Protocols

Product Specific Protocols
WB protocol for DDX46 antibody 85065-3-RRDownload protocol
IF protocol for DDX46 antibody 85065-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols