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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DDX54 Monoclonal antibody

DDX54 Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1H9F4

Cat no : 66664-1-Ig

Synonyms

EC:3.6.4.13, DP97, DDX 54, ATP-dependent RNA helicase DP97, ATP-dependent RNA helicase DDX54

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using DDX54 Monoclonal antibody (66664-1-Ig)

    WB analysis using 66664-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66664-1-Ig (DDX54 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

  • Western Blot (WB) analysis of various lysates using DDX54 Monoclonal antibody (66664-1-Ig)

    WB analysis using 66664-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66664-1-Ig (DDX54 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using DDX54 Monoclonal antibody (66664-1-Ig)

    IF Staining of PC-3 using 66664-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using DDX54 antibody (66664-1-Ig, Clone: 1H9F4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using DDX54 Monoclonal antibody (66664-1-Ig)

    IF Staining of PC-3 using 66664-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using DDX54 antibody (66664-1-Ig, Clone: 1H9F4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of PC-3 cells using DDX54 Monoclonal antibody (66664-1-Ig)

    FC experiment of PC-3 using 66664-1-Ig

    1X10^6 PC-3 cells were intracellularly stained with 0.4 ug Anti-Human DDX54 (66664-1-Ig, Clone:1H9F4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive WB detected inHepG2 cells, LNCaP cells, HeLa cells, Jurkat cells, K-562 cells, PC-3 cells, U-251 cells, U-87 MG cells
Positive IF/ICC detected inPC-3 cells
Positive FC (Intra) detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66664-1-Ig targets DDX54 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen DDX54 fusion protein Ag25289 相同性解析による交差性が予測される生物種
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 54
Calculated molecular weight 98 kDa
Observed molecular weight 98 kDa
GenBank accession numberBC156669
Gene symbol DDX54
Gene ID (NCBI) 79039
RRIDAB_2882019
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DDX54, also named as ATP-dependent RNA helicase DDX54, is a 881 amino acid protein, which contains 1 helicase ATP-binding domain and belongs to the DEAD box helicase family. DDX54/DBP10 subfamily. DDX54 localizes in nucleus and Interacts in a hormone-dependent manner with nuclear receptors. DDX54 has RNA-dependent ATPase activity and represses the transcriptional activity of nuclear receptors.

Protocols

Product Specific Protocols
WB protocol for DDX54 antibody 66664-1-IgDownload protocol
IF protocol for DDX54 antibody 66664-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Front Oncol

DDX54 Plays a Cancerous Role Through Activating P65 and AKT Signaling Pathway in Colorectal Cancer.

Authors - Yi Yu
View Article