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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated DDX54 Monoclonal antibody

DDX54 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1H9F4

Cat no : CL594-66664

Synonyms

EC:3.6.4.13, DP97, DDX 54, ATP-dependent RNA helicase DP97, ATP-dependent RNA helicase DDX54

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using CoraLite®594-conjugated DDX54 Monoclonal antibody (CL594-66664)

    IF Staining of PC-3 using CL594-66664

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using CoraLite®594 DDX54 antibody (CL594-66664, Clone: 1H9F4 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of PC-3 cells using CoraLite®594-conjugated DDX54 Monoclonal antibody (CL594-66664)

    FC experiment of PC-3 using CL594-66664

    1X10^6 PC-3 cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human DDX54 (CL594-66664, Clone:1H9F4) (red), or 0.8 ug CoraLite®594 Mouse IgG2a Isotype Control (C1.18.4) (CL594-65208, Clone: C1.18.4) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive IF/ICC detected inPC-3 cells
Positive FC (Intra) detected inPC-3 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66664 targets DDX54 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen DDX54 fusion protein Ag25289 相同性解析による交差性が予測される生物種
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 54
Calculated molecular weight 98 kDa
Observed molecular weight 98 kDa
GenBank accession numberBC156669
Gene symbol DDX54
Gene ID (NCBI) 79039
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DDX54, also named as ATP-dependent RNA helicase DDX54, is a 881 amino acid protein, which contains 1 helicase ATP-binding domain and belongs to the DEAD box helicase family. DDX54/DBP10 subfamily. DDX54 localizes in nucleus and Interacts in a hormone-dependent manner with nuclear receptors. DDX54 has RNA-dependent ATPase activity and represses the transcriptional activity of nuclear receptors.

Protocols

Product Specific Protocols
IF protocol for CL594 DDX54 antibody CL594-66664Download protocol
FC protocol for CL594 DDX54 antibody CL594-66664Download protocol
Standard Protocols
Click here to view our Standard Protocols