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Validation Data Gallery

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  • Flow cytometry (FC) experiment of HeLa cells using CoraLite®555-conjugated DEK Monoclonal antibody (CL555-66194)

    FC experiment of HeLa using CL555-66194

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human DEK (CL555-66194, Clone:5E3A6) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  • Featured Product
  • KD/KO Validated

CoraLite®555-conjugated DEK Monoclonal antibody

DEK Monoclonal Antibody for FC (Intra)
Cat No. CL555-66194
Clone No.5E3A6

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

FC (Intra)

Synonyms

D6S231E, DEK, DEK oncogene, Protein DEK

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®555
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL555-66194 targets DEK in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen DEK fusion protein Ag8225 相同性解析による交差性が予測される生物種
Full Name DEK oncogene
Calculated molecular weight 43 kDa
Observed molecular weight 40-50 kDa
GenBank accession numberBC035259
Gene Symbol DEK
Gene ID (NCBI) 7913
RRIDAB_2919678
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission maxima wavelengths557 nm / 570 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP35659
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Dek oncogene(DEK) is first recognized in a fusion with CAN nucleoporin protein, and it's a also autoantigen in patients with pquciarticular onset juvenile rheumatoid arthritis. As a site-specific binding protein, DEK may involve in transcriptional regulation and signal transdcution. Analysis by agarose gel celctrophoresis, DEK showed to reduce the efficiency with which chromation is replicated. It's one of the first components of the splicing complex, which remains associate with spliced exons dependent on prior splicing of pre-mRNA.

Protocols

Product Specific Protocols
FC protocol for CL555 DEK antibody CL555-66194Download protocol
Standard Protocols
Click here to view our Standard Protocols