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CoraLite® Plus 488-conjugated DGKA Polyclonal antibody
DGKA Polyclonal Antibody for IF/ICC, FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF/ICC, FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
Cat no : CL488-11547
Synonyms
Validation Data Gallery
Tested Applications
Positive IF/ICC detected in | Jurkat cells |
Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
Application | Dilution |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL488-11547 targets DGKA in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | DGKA fusion protein Ag2075 相同性解析による交差性が予測される生物種 |
Full Name | diacylglycerol kinase, alpha 80kDa |
Calculated molecular weight | 735 aa, 83 kDa |
Observed molecular weight | 83 kDa |
GenBank accession number | BC023523 |
Gene symbol | DGKA |
Gene ID (NCBI) | 1606 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
DGKA(Diacylglycerol kinase alpha) is also named as DAGK, DAGK1 and belongs to the eukaryotic diacylglycerol kinase family,which is suggested to attenuate diacylglycerol-induced cell responses through the phosphorylation of this second messenger to phosphatidic acid.DGKA regulates the secretion of lethal exosomes bearing Fas ligand during activation-induced cell death of T lymphocytes.
Protocols
Product Specific Protocols | |
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IF protocol for CL Plus 488 DGKA antibody CL488-11547 | Download protocol |
FC protocol for CL Plus 488 DGKA antibody CL488-11547 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |