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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using CoraLite® Plus 488-conjugated DGKA Polyclonal anti (CL488-11547)

    IF Staining of Jurkat using CL488-11547

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CoraLite® Plus 488 DGKA antibody (CL488-11547) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated DGKA Polyclonal anti (CL488-11547)

    FC experiment of HeLa using CL488-11547

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human DGKA (CL488-11547) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated DGKA Polyclonal antibody

DGKA Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-11547

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Synonyms

EC:2.7.1.107, DGK-alpha, DGK alpha, DAGK1, DAGK

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inJurkat cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-11547 targets DGKA in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DGKA fusion protein Ag2075 相同性解析による交差性が予測される生物種
Full Name diacylglycerol kinase, alpha 80kDa
Calculated molecular weight 735 aa, 83 kDa
Observed molecular weight 83 kDa
GenBank accession numberBC023523
Gene Symbol DGKA
Gene ID (NCBI) 1606
RRIDAB_3083904
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP23743
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DGKA(Diacylglycerol kinase alpha) is also named as DAGK, DAGK1 and belongs to the eukaryotic diacylglycerol kinase family,which is suggested to attenuate diacylglycerol-induced cell responses through the phosphorylation of this second messenger to phosphatidic acid.DGKA regulates the secretion of lethal exosomes bearing Fas ligand during activation-induced cell death of T lymphocytes.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 DGKA antibody CL488-11547Download protocol
FC protocol for CL Plus 488 DGKA antibody CL488-11547Download protocol
Standard Protocols
Click here to view our Standard Protocols