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Validation Data Gallery

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  • Western Blot (WB) analysis of LNCaP cells using DHODH Monoclonal antibody (67977-1-Ig)

    WB analysis of LNCaP using 67977-1-Ig

    LNCaP cells were subjected to SDS PAGE followed by western blot with 67977-1-Ig (DHODH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HSC-T6 cells using DHODH Monoclonal antibody (67977-1-Ig)

    WB analysis of HSC-T6 using 67977-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67977-1-Ig (DHODH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using DHODH Monoclonal antibody (67977-1-Ig)

    WB analysis of NIH/3T3 using 67977-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67977-1-Ig (DHODH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of 4T1 cells using DHODH Monoclonal antibody (67977-1-Ig)

    WB analysis of 4T1 using 67977-1-Ig

    4T1 cells were subjected to SDS PAGE followed by western blot with 67977-1-Ig (DHODH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using DHODH Monoclonal antibody (67977-1-Ig)

    WB analysis of HeLa using 67977-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 67977-1-Ig (DHODH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using DHODH Monoclonal antibody (67977-1-Ig)

    WB analysis of HEK-293 using 67977-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67977-1-Ig (DHODH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using DHODH Monoclonal antibody (67977-1-Ig)

    WB analysis of K-562 using 67977-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 67977-1-Ig (DHODH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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DHODH Monoclonal antibody

DHODH Monoclonal Antibody for WB, ELISA
Cat No. 67977-1-Ig
Clone No.2B2C6
Publications(2)

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat and More (1)

Applications

WB, ELISA

Synonyms

DHOdehase, DHODH, dihydroorotate dehydrogenase, Dihydroorotate oxidase

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inLNCaP cells, HeLa cells, HEK-293 cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

67977-1-Ig targets DHODH in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivitymouse, monkey
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen DHODH fusion protein Ag6649 相同性解析による交差性が予測される生物種
Full Name dihydroorotate dehydrogenase
Calculated molecular weight 43 kDa
Observed molecular weight 43 kDa
GenBank accession numberBC065245
Gene Symbol DHODH
Gene ID (NCBI) 1723
RRIDAB_2918726
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ02127
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

DHODH(Dihydroorotate dehydrogenase) catalyzes the fourth enzymatic step in de novo pyrimidine biosynthesis.DHO dehydrogenase is a monofunctional protein which, in most eukaryotic organisms, is located on the outer surface of the inner mitochondrial membrane.Defects in DHODH are the cause of postaxial acrofacial dysostosis (POADS).

Protocols

Product Specific Protocols
WB protocol for DHODH antibody 67977-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
monkeyWB

Vet Res

Vidofludimus inhibits porcine reproductive and respiratory syndrome virus infection by targeting dihydroorotate dehydrogenase

Authors - Yuanqi Yang
View Article
mouseWB

ACS Nano

Engineering Biodegradable Hollow Silica/Iron Composite Nanozymes for Breast Tumor Treatment through Activation of the "Ferroptosis Storm"

Authors - Panpan Xue
View Article