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Validation Data Gallery

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  • Western Blot (WB) analysis of LNCaP cells using DHODH Monoclonal antibody (67977-1-Ig)

    WB analysis of LNCaP using 67977-1-Ig (same clone as 67977-1-PBS)

    LNCaP cells were subjected to SDS PAGE followed by western blot with 67977-1-Ig (DHODH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67977-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using DHODH Monoclonal antibody (67977-1-Ig)

    WB analysis of HSC-T6 using 67977-1-Ig (same clone as 67977-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67977-1-Ig (DHODH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67977-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using DHODH Monoclonal antibody (67977-1-Ig)

    WB analysis of NIH/3T3 using 67977-1-Ig (same clone as 67977-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67977-1-Ig (DHODH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67977-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of 4T1 cells using DHODH Monoclonal antibody (67977-1-Ig)

    WB analysis of 4T1 using 67977-1-Ig (same clone as 67977-1-PBS)

    4T1 cells were subjected to SDS PAGE followed by western blot with 67977-1-Ig (DHODH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67977-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using DHODH Monoclonal antibody (67977-1-Ig)

    WB analysis of HeLa using 67977-1-Ig (same clone as 67977-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 67977-1-Ig (DHODH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67977-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using DHODH Monoclonal antibody (67977-1-Ig)

    WB analysis of HEK-293 using 67977-1-Ig (same clone as 67977-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67977-1-Ig (DHODH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67977-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using DHODH Monoclonal antibody (67977-1-Ig)

    WB analysis of K-562 using 67977-1-Ig (same clone as 67977-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 67977-1-Ig (DHODH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67977-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50416-1

    Cytometric bead array standard curve of MP50416-1

    Cytometric bead array standard curve of MP50416-1, DHODH Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67977-2-PBS. Detection antibody: 67977-1-PBS. Standard:Ag6649. Range: 1.563-100 ng/mL.

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DHODH Monoclonal antibody, PBS Only (Detector)

DHODH Monoclonal Antibody for WB, Cytometric bead array, Indirect ELISA
Cat No. 67977-1-PBS
Clone No.2B2C6

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, Cytometric bead array, Indirect ELISA

Synonyms

PYRD, EC:1.3.5.2, Dihydroorotate oxidase, Dihydroorotate dehydrogenase (quinone), mitochondrial, dihydroorotate dehydrogenase

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67977-1-PBS targets DHODH as part of a matched antibody pair:

MP50416-1: 67977-2-PBS capture and 67977-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen DHODH fusion protein Ag6649 相同性解析による交差性が予測される生物種
Full Name dihydroorotate dehydrogenase
Calculated molecular weight 43 kDa
Observed molecular weight 43 kDa
GenBank accession numberBC065245
Gene Symbol DHODH
Gene ID (NCBI) 1723
RRIDAB_2918726
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ02127
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

DHODH(Dihydroorotate dehydrogenase) catalyzes the fourth enzymatic step in de novo pyrimidine biosynthesis.DHO dehydrogenase is a monofunctional protein which, in most eukaryotic organisms, is located on the outer surface of the inner mitochondrial membrane.Defects in DHODH are the cause of postaxial acrofacial dysostosis (POADS).