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Validation Data Gallery

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  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated DHX37 Monoclonal ant (CL488-67853)

    FC experiment of HeLa using CL488-67853

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human DHX37 (CL488-67853, Clone:2B12D9) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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CoraLite® Plus 488-conjugated DHX37 Monoclonal antibody

DHX37 Monoclonal Antibody for FC (Intra)
Cat No. CL488-67853
Clone No.2B12D9

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

FC (Intra)

Synonyms

DDX37, DEAH box protein 37, DHX37, FLJ41974, KIAA1517

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67853 targets DHX37 in FC (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen DHX37 fusion protein Ag29909 相同性解析による交差性が予測される生物種
Full Name DEAH (Asp-Glu-Ala-His) box polypeptide 37
Calculated molecular weight 1157 aa, 130 kDa
Observed molecular weight130-140 kDa
GenBank accession numberBC037964
Gene Symbol DHX37
Gene ID (NCBI) 57647
RRIDAB_2923803
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8IY37
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DHX37, also known as DDX37, KIAA1517. It is an ATP-bound RNA helicase that plays a role in the maturation of small ribosomal subunits in ribosomal biogenesis (PMID:30582406). The presence of DHX37 in early pre-ribosomal particles was monitored by a quality control pathway, and failure to recruit DHX37 resulted in pre-RRNA degradation. DHX37 binds directly to U3 small nucleolar RNA (snoRNA), and the catalytic activity of the helicase is required for dissociation of U3 small nucleolar RNA from the pre-ribosomal complex.In addition, it plays a role in early testicular development (PMID:31287541, PMID:31337883). It may also have an effect on brain development (PubMed:31256877). It is expressed in the fallopian tubes, ovaries, uterus, testes and brain (PMID: 31256877,PMID: 31337883).

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 DHX37 antibody CL488-67853Download protocol
Standard Protocols
Click here to view our Standard Protocols