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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated DHX9 Monoclonal antibody

DHX9 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B12C10

Cat no : CL488-67153

Synonyms

LKP, EC:3.6.4.13, DExH-box helicase 9, DDX9, ATP-dependent RNA helicase A

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated DHX9 Monoclonal anti (CL488-67153)

    IF Staining of HepG2 using CL488-67153

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 DHX9 antibody (CL488-67153, Clone: 1B12C10 ) at dilution of 1:400.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated DHX9 Monoclonal anti (CL488-67153)

    IF Staining of HepG2 using CL488-67153

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 DHX9 antibody (CL488-67153, Clone: 1B12C10 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of Jurkat cells using CoraLite® Plus 488-conjugated DHX9 Monoclonal anti (CL488-67153)

    FC experiment of Jurkat using CL488-67153

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human DHX9 (CL488-67153, Clone:1B12C10) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inJurkat cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67153 targets DHX9 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen DHX9 fusion protein Ag12104 相同性解析による交差性が予測される生物種
Full Name DEAH (Asp-Glu-Ala-His) box polypeptide 9
Calculated molecular weight 1270 aa, 141 kDa
Observed molecular weight 140 kDa
GenBank accession numberBC014246
Gene symbol DHX9
Gene ID (NCBI) 1660
RRIDAB_2919429
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RNA helicases play important roles in transcription, RNA processing, translation, and RNA replication. DEAD box proteins are putative RNA helicases that have a characteristic Asp-Glu-Ala-Asp (DEAD) box as 1 of 8 highly conserved sequence motifs. DHX9 a member of the DEAH family of proteins, which possess a double-stranded RNA-binding domain (dsRBD) and a helicase domain [PMID:20569003]. It unwinds double-stranded DNA and RNA in a 3' to 5' direction. Alteration of secondary structure of DHX9 may subsequently influence interactions with proteins or other nucleic acids. It is also a component of the CRD-mediated complex that promotes MYC mRNA stability. In addition, it is involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2[PMID: 19029303, 22190748].

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 DHX9 antibody CL488-67153Download protocol
Standard Protocols
Click here to view our Standard Protocols