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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Smac/DIABLO Monoclonal antibody

Smac/DIABLO Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2F12C9

Cat no : 68480-1-Ig

Synonyms

DIABLO, Diablo IAP-binding mitochondrial protein, cleaved form, Diablo IAP-binding mitochondrial protein, Diablo homolog, mitochondrial, DFNA64

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DIABLO Monoclonal antibody (68480-1-Ig)

    WB analysis using 68480-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68480-1-Ig (DIABLO antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of various lysates using DIABLO Monoclonal antibody (68480-1-Ig)

    WB analysis using 68480-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68480-1-Ig (DIABLO antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inU2OS cells, K-562 cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, HSC-T6 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68480-1-Ig targets Smac/DIABLO in WB, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Smac/DIABLO fusion protein Ag32774 相同性解析による交差性が予測される生物種
Full Name diablo homolog (Drosophila)
Calculated molecular weight 27 kDa
Observed molecular weight 20 kDa
GenBank accession numberBC011909
Gene symbol DIABLO
Gene ID (NCBI) 56616
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Smac/DIABLO is a mitochondrial protein that promotes apoptosis by neutralizing members of the IAP family. SMAC/DIABLO is ubiquitously expressed in normal tissues, and the expression of SMAC messenger RNA (mRNA) in adult testis is the highest .Smac/DIABLO encodes a 239 amino acid protein (~27 kDa). The first 55 amino acids are a mitochondrial targeting signal peptide that is cleaved to generate mature Smac-DIABLO (~21 kDa). When released from mitochondria, Smac-DIABLO acts as a natural antagonist of inhibitor of apoptosis proteins (IAPs). Smac-DIABLO antagonistic activity is based on its N-terminal tetrapeptide (AVPI) that binds baculoviral IAP repeat (BIR) domains of IAPs, releasing their inhibitory effects on both initiator and effector caspases, thus promoting cell death. (PMID: 32325691, PMID: 32575872,PMID: 25650938)

Protocols

Product Specific Protocols
WB protocol for Smac/DIABLO antibody 68480-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols