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Host
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Smac/DIABLO Monoclonal antibody, PBS Only (Detector)

Smac/DIABLO Monoclonal Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2F12C9

Cat no : 68480-1-PBS

Synonyms

DIABLO, Diablo IAP-binding mitochondrial protein, cleaved form, Diablo IAP-binding mitochondrial protein, Diablo homolog, mitochondrial, DFNA64

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using Smac/DIABLO Monoclonal antibody (68480-1-Ig)

    WB analysis using 68480-1-Ig (same clone as 68480-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68480-1-Ig (DIABLO antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68480-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Smac/DIABLO Monoclonal antibody (68480-1-Ig)

    WB analysis using 68480-1-Ig (same clone as 68480-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68480-1-Ig (DIABLO antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68480-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50334-2

    Cytometric bead array standard curve of MP50334-2

    Cytometric bead array standard curve of MP50334-2, DIABLO Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68480-4-PBS. Detection antibody: 68480-1-PBS. Standard:Ag32774. Range: 1.563-100 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68480-1-PBS targets Smac/DIABLO as part of a matched antibody pair:

MP50334-2: 68480-4-PBS capture and 68480-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Smac/DIABLO fusion protein Ag32774 相同性解析による交差性が予測される生物種
Full Name diablo homolog (Drosophila)
Calculated molecular weight 27 kDa
Observed molecular weight 20 kDa
GenBank accession numberBC011909
Gene symbol DIABLO
Gene ID (NCBI) 56616
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Smac/DIABLO is a mitochondrial protein that promotes apoptosis by neutralizing members of the IAP family. SMAC/DIABLO is ubiquitously expressed in normal tissues, and the expression of SMAC messenger RNA (mRNA) in adult testis is the highest .Smac/DIABLO encodes a 239 amino acid protein (~27 kDa). The first 55 amino acids are a mitochondrial targeting signal peptide that is cleaved to generate mature Smac-DIABLO (~21 kDa). When released from mitochondria, Smac-DIABLO acts as a natural antagonist of inhibitor of apoptosis proteins (IAPs). Smac-DIABLO antagonistic activity is based on its N-terminal tetrapeptide (AVPI) that binds baculoviral IAP repeat (BIR) domains of IAPs, releasing their inhibitory effects on both initiator and effector caspases, thus promoting cell death. (PMID: 32325691, PMID: 32575872,PMID: 25650938)