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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Smac/DIABLO Polyclonal antibody

Smac/DIABLO Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-10434

Synonyms

DIABLO, DFNA64, Direct IAP-binding protein with low pI, Diablo IAP-binding mitochondrial protein, cleaved form, Diablo IAP-binding mitochondrial protein

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated DIABLO Polyclonal an (CL488-10434)

    IF Staining of HeLa using CL488-10434

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 DIABLO antibody (CL488-10434) at dilution of 1:100.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated DIABLO Polyclonal an (CL488-10434)

    FC experiment of HeLa using CL488-10434

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human DIABLO (CL488-10434) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-10434 targets Smac/DIABLO in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Smac/DIABLO fusion protein Ag0696 相同性解析による交差性が予測される生物種
Full Name diablo homolog (Drosophila)
Calculated molecular weight 27 kDa
Observed molecular weight 20 kDa
GenBank accession numberBC011909
Gene symbol DIABLO
Gene ID (NCBI) 56616
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Smac/DIABLO is a mitochondrial protein that promotes apoptosis by neutralizing members of the IAP family. SMAC/DIABLO is ubiquitously expressed in normal tissues, and the expression of SMAC messenger RNA (mRNA) in adult testis is the highest .Smac/DIABLO encodes a 239 amino acid protein (~27 kDa). The first 55 amino acids are a mitochondrial targeting signal peptide that is cleaved to generate mature Smac-DIABLO (~21 kDa). When released from mitochondria, Smac-DIABLO acts as a natural antagonist of inhibitor of apoptosis proteins (IAPs). Smac-DIABLO antagonistic activity is based on its N-terminal tetrapeptide (AVPI) that binds baculoviral IAP repeat (BIR) domains of IAPs, releasing their inhibitory effects on both initiator and effector caspases, thus promoting cell death. (PMID: 32325691, PMID: 32575872,PMID: 25650938)

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Smac/DIABLO antibody CL488-10434Download protocol
FC protocol for CL Plus 488 Smac/DIABLO antibody CL488-10434Download protocol
Standard Protocols
Click here to view our Standard Protocols