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DIAPH3 Polyclonal antibody

Name: DIAPH3 Polyclonal antibody
Brand: Proteintech
SKU: 14342-1-AP

DIAPH3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 14342-1-AP

Synonyms

MDia2, DRF3, Diaphanous-related formin-3, DIAP3

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis of HeLa using 14342-1-AP
WB result of DIAPH3 antibody (14342-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DIAPH3 transfected HeLa cells.
WB analysis of HeLa using 14342-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 14342-1-AP (DIAPH3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 14342-1-AP
WB result of DIAPH3 antibody (14342-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DIAPH3 transfected HEK-293 cells.
WB analysis of DU 145 using 14342-1-AP
DU 145 cells were subjected to SDS PAGE followed by western blot with 14342-1-AP (DIAPH3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 14342-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 14342-1-AP (DIAPH3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 14342-1-AP
IP result of anti-DIAPH3 (IP:14342-1-AP, 4ug; Detection:14342-1-AP 1:500) with HeLa cells lysate 2000ug.
IHC staining of human cervical cancer using 14342-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14342-1-AP (DIAPH3 Antibody) at dilution of 1:50.
IHC staining of mouse testis using 14342-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14342-1-AP (DIAPH3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat testis using 14342-1-AP
Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 14342-1-AP (DIAPH3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 14342-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14342-1-AP (DIAPH3 Antibody) at dilution of 1:50.
IHC staining of human prostate cancer using 14342-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14342-1-AP (DIAPH3 Antibody) at dilution of 1:50.
IHC staining of human cervical cancer using 14342-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14342-1-AP (DIAPH3 Antibody) at dilution of 1:50.
IF Staining of HeLa using 14342-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using DIAPH3 antibody (14342-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of HeLa using 14342-1-AP
Immunofluorescent analysis of HeLa cells using 14342-1-AP (DIAPH3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 14342-1-AP

Size/Concentration

ご購入について

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, DU 145 cells, HEK-293 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	mouse testis tissue, human cervical cancer tissue, rat testis tissue, human prostate cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 7 publications below
WB	See 18 publications below
IHC	See 3 publications below
IF	See 6 publications below
IP	See 1 publications below

Product Information

14342-1-AP targets DIAPH3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	DIAPH3 fusion protein Ag5656 相同性解析による交差性が予測される生物種
Full Name	diaphanous homolog 3 (Drosophila)
Calculated molecular weight	137 kDa
Observed molecular weight	125-137 kDa
GenBank accession number	BC048963
Gene symbol	DIAPH3
Gene ID (NCBI)	81624
RRID	AB_2092930
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

DIAPH3 is also refered as diaphanous-related formin-3 which binds to GTP-bound form of Rho and to profilin, acts in a Rho-dependent manner to recruit profilin to the membrane. It is required for cytokinesis, stress fiber formation, and transcriptional activation of the serum response factor. Seven isoforms of DIAPH3 exist due to the alternative splicing. Recently it has been reported that dimerization and actin assembly activity of DIAPH3 isoforms 1 and 7 are essential for induction of specific cell protrusions. It may propose that isoform-selective actin assembly by DIAPH3 exerts specific and differentially regulated functions during cell adhesion and motility.

Protocols

Product Specific Protocols
WB protocol for DIAPH3 antibody 14342-1-AP	Download protocol
IHC protocol for DIAPH3 antibody 14342-1-AP	Download protocol
IF protocol for DIAPH3 antibody 14342-1-AP	Download protocol
IP protocol for DIAPH3 antibody 14342-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse,human	IP	Blood Membrane skeleton modulates erythroid proteome remodeling and organelle clearance. Authors - Yijie Liu KO Validated View Article
human	IF	Sci Adv Recessive NOS1AP variants impair actin remodeling and cause glomerulopathy in humans and mice. Authors - Amar J Majmundar View Article
human	IHC,WB	Aging (Albany NY) The pan-cancer analysis identified DIAPH3 as a diagnostic biomarker of clinical cancer Authors - Xiaowei Chen View Article
human	WB	Cell Res Differing and isoform-specific roles for the formin DIAPH3 in plasma membrane blebbing and filopodia formation. Authors - Stastna Jana J KD Validated View Article
human	WB,IHC,IF	EMBO J TFAM loss induces nuclear actin assembly upon mDia2 malonylation to promote liver cancer metastasis. Authors - Qichao Huang View Article
human	WB	Elife Generation of stress fibers through myosin-driven reorganization of the actin cortex Authors - Jaakko I Lehtimäki KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DIAPH3 Polyclonal antibody

DIAPH3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis of HeLa using 14342-1-AP

WB analysis of HeLa using 14342-1-AP

WB analysis of HEK-293 using 14342-1-AP

WB analysis of DU 145 using 14342-1-AP

WB analysis of HEK-293 using 14342-1-AP

IP experiment of HeLa using 14342-1-AP

IHC staining of human cervical cancer using 14342-1-AP

IHC staining of mouse testis using 14342-1-AP

IHC staining of rat testis using 14342-1-AP

IHC staining of human prostate cancer using 14342-1-AP

IHC staining of human prostate cancer using 14342-1-AP

IHC staining of human cervical cancer using 14342-1-AP

IF Staining of HeLa using 14342-1-AP

IF Staining of HeLa using 14342-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Membrane skeleton modulates erythroid proteome remodeling and organelle clearance.

Recessive NOS1AP variants impair actin remodeling and cause glomerulopathy in humans and mice.

The pan-cancer analysis identified DIAPH3 as a diagnostic biomarker of clinical cancer

Differing and isoform-specific roles for the formin DIAPH3 in plasma membrane blebbing and filopodia formation.

TFAM loss induces nuclear actin assembly upon mDia2 malonylation to promote liver cancer metastasis.

Generation of stress fibers through myosin-driven reorganization of the actin cortex