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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated DIAPH3 Polyclonal antibody

DIAPH3 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-14342

Synonyms

diap3, DIAPH3, Diaphanous related formin 3, DKFZp434C0931, DKFZp686A13178, DRF3, FLJ34705, mDia2, Protein diaphanous homolog 3

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated DIAPH3 Polyclonal an (CL488-14342)

    IF Staining of HeLa using CL488-14342

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 DIAPH3 antibody (CL488-14342) at dilution of 1:200.

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Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-14342 targets DIAPH3 in IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DIAPH3 fusion protein Ag5656 相同性解析による交差性が予測される生物種
Full Name diaphanous homolog 3 (Drosophila)
Calculated molecular weight 137 kDa
Observed molecular weight 125-137 kDa
GenBank accession numberBC048963
Gene symbol DIAPH3
Gene ID (NCBI) 81624
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DIAPH3 is also refered as diaphanous-related formin-3 which binds to GTP-bound form of Rho and to profilin, acts in a Rho-dependent manner to recruit profilin to the membrane. It is required for cytokinesis, stress fiber formation, and transcriptional activation of the serum response factor. Seven isoforms of DIAPH3 exist due to the alternative splicing. Recently it has been reported that dimerization and actin assembly activity of DIAPH3 isoforms 1 and 7 are essential for induction of specific cell protrusions. It may propose that isoform-selective actin assembly by DIAPH3 exerts specific and differentially regulated functions during cell adhesion and motility.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 DIAPH3 antibody CL488-14342Download protocol
Standard Protocols
Click here to view our Standard Protocols