Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 16431-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16431-1-AP (DLD antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 16431-1-AP
WB result of DLD antibody (16431-1-AP; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DLD transfected HeLa cells.
WB analysis using 16431-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16431-1-AP (DLD antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 16431-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 16431-1-AP (DLD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 16431-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 16431-1-AP (DLD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 16431-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 16431-1-AP (DLD Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis using 16431-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16431-1-AP (DLD antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IP experiment of mouse liver using 16431-1-AP
IP result of anti-DLD (IP:16431-1-AP, 4ug; Detection:16431-1-AP 1:1000) with mouse liver tissue lysate 6000ug.
IHC staining of human liver cancer using 16431-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16431-1-AP (DLD antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 16431-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16431-1-AP (DLD antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human heart using 16431-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 16431-1-AP (DLD antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human heart using 16431-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 16431-1-AP (DLD antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human kidney using 16431-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 16431-1-AP (DLD antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human kidney using 16431-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 16431-1-AP (DLD antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human testis using 16431-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 16431-1-AP (DLD antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human testis using 16431-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 16431-1-AP (DLD antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human liver using 16431-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 16431-1-AP (DLD antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human liver using 16431-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 16431-1-AP (DLD antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human liver cancer using 16431-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16431-1-AP (DLD antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 16431-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16431-1-AP (DLD antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 16431-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DLD antibody (16431-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).
IF Staining of HepG2 using 16431-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DLD antibody (16431-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

Featured Product
KD/KO Validated

DLD Polyclonal antibody

DLD Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 16431-1-AP

Publications(36)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

DLDH, EC:1.8.1.4, GCSL, LAD, PHE3

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (36)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HepG2 cells, HeLa cells, human brain tissue, human liver tissue, L02 cells, mouse heart tissue, mouse liver tissue, rat liver tissue, rat heart tissue
Positive IP detected in	mouse liver tissue
Positive IHC detected in	human liver cancer tissue, human heart tissue, human kidney tissue, human testis tissue, human liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:10000-1:500000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 31 publications below
IHC	See 5 publications below
IF	See 2 publications below
IP	See 2 publications below
CoIP	See 1 publications below

Product Information

16431-1-AP targets DLD in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag9729 Product name: Recombinant human DLD protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 160-509 aa of BC018696 Sequence: NQVTATKADGGTQVIDTKNILIATGSEVTPFPGITIDEDTIVSSTGALSLKKVPEKMVVIGAGVIGVELGSVWQRLGADVTAVEFLGHVGGVGIDMEISKNFQRILQKQGFKFKLNTKVTGATKKSDGKIDVSIEAASGGKAEVITCDVLLVCIGRRPFTKNLGLEELGIELDPRGRIPVNTRFQTKIPNIYAIGDVVAGPMLAHKAEDEGIICVEGMAGGAVHIDYNCVPSVIYTHPEVAWVGKSEEQLKEEGIEYKVGKFPFAANSRAKTNADTDGMVKILGQKSTDRVLGAHILGPGAGEMVNEAALALEYGASCEDIARVCHAHPTLSEAFREANLAASFGKSINF 相同性解析による交差性が予測される生物種
Full Name	dihydrolipoamide dehydrogenase
Calculated molecular weight	509 aa, 54 kDa
Observed molecular weight	56 kDa
GenBank accession number	BC018696
Gene Symbol	DLD
Gene ID (NCBI)	1738
RRID	AB_2091888
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P09622
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

DLD(Dihydrolipoyl dehydrogenase, mitochondrial) is also named as GCSL, LAD, PHE3 and belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family. It catalyzes the oxidation of dihydrolipoamide, hE3 uses two molecules : non-covalently bound FAD and a transiently bound substrate, NAD+. DLD is involved in the hyperactivation of spermatazoa during capacitation and in the spermatazoal acrosome reaction.

Protocols

Product Specific Protocols
IF protocol for DLD antibody 16431-1-AP	Download protocol
IHC protocol for DLD antibody 16431-1-AP	Download protocol
IP protocol for DLD antibody 16431-1-AP	Download protocol
WB protocol for DLD antibody 16431-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Cell Res Hypoxia induces mitochondrial protein lactylation to limit oxidative phosphorylation Authors - Yunzi Mao View Article
		Science Copper induces cell death by targeting lipoylated TCA cycle proteins. Authors - Peter Tsvetkov View Article
mouse	WB	Nat Cell Biol RIP3 targets pyruvate dehydrogenase complex to increase aerobic respiration in TNF-induced necroptosis Authors - KO Validated View Article
human	WB	J Hepatol OGDHL silencing promotes hepatocellular carcinoma by reprogramming glutamine metabolism. Authors - Weiqi Dai View Article
human	WB	Nat Commun Blocking glycine utilization inhibits multiple myeloma progression by disrupting glutathione balance. Authors - Jiliang Xia KD Validated View Article
human	WB	Redox Biol Transketolase in human Müller cells is critical to resist light stress through the pentose phosphate and NRF2 pathways. Authors - Yingying Chen View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 16431-1-AP

WB analysis of HeLa using 16431-1-AP

WB analysis using 16431-1-AP

WB analysis of human liver using 16431-1-AP

WB analysis of human brain using 16431-1-AP

WB analysis of HeLa using 16431-1-AP

WB analysis using 16431-1-AP

IP experiment of mouse liver using 16431-1-AP

IHC staining of human liver cancer using 16431-1-AP

IHC staining of human liver cancer using 16431-1-AP

IHC staining of human heart using 16431-1-AP

IHC staining of human heart using 16431-1-AP

IHC staining of human kidney using 16431-1-AP

IHC staining of human kidney using 16431-1-AP

IHC staining of human testis using 16431-1-AP

IHC staining of human testis using 16431-1-AP

IHC staining of human liver using 16431-1-AP

IHC staining of human liver using 16431-1-AP

IHC staining of human liver cancer using 16431-1-AP

IHC staining of human liver cancer using 16431-1-AP

IF Staining of HepG2 using 16431-1-AP

IF Staining of HepG2 using 16431-1-AP

DLD Polyclonal antibody

DLD Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Hypoxia induces mitochondrial protein lactylation to limit oxidative phosphorylation

Copper induces cell death by targeting lipoylated TCA cycle proteins.

RIP3 targets pyruvate dehydrogenase complex to increase aerobic respiration in TNF-induced necroptosis

OGDHL silencing promotes hepatocellular carcinoma by reprogramming glutamine metabolism.

Blocking glycine utilization inhibits multiple myeloma progression by disrupting glutathione balance.

Transketolase in human Müller cells is critical to resist light stress through the pentose phosphate and NRF2 pathways.