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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated DLD Polyclonal antibody

DLD Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-16431

Synonyms

dihydrolipoamide dehydrogenase, DLD, DLDH, E3, GCSL, LAD, PHE3

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated DLD Polyclonal antib (CL488-16431)

    IF Staining of HepG2 using CL488-16431

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 DLD antibody (CL488-16431) at dilution of 1:100.

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Tested Applications

Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-16431 targets DLD in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DLD fusion protein Ag9729 相同性解析による交差性が予測される生物種
Full Name dihydrolipoamide dehydrogenase
Calculated molecular weight 509 aa, 54 kDa
Observed molecular weight 56 kDa
GenBank accession numberBC018696
Gene symbol DLD
Gene ID (NCBI) 1738
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DLD(Dihydrolipoyl dehydrogenase, mitochondrial) is also named as GCSL, LAD, PHE3 and belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family. It catalyzes the oxidation of dihydrolipoamide, hE3 uses two molecules : non-covalently bound FAD and a transiently bound substrate, NAD+. DLD is involved in the hyperactivation of spermatazoa during capacitation and in the spermatazoal acrosome reaction.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 DLD antibody CL488-16431Download protocol
Standard Protocols
Click here to view our Standard Protocols