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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using CoraLite® Plus 488-conjugated DMAP1 Monoclonal ant (CL488-67183)

    IF Staining of HEK-293 using CL488-67183

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CoraLite® Plus 488 DMAP1 antibody (CL488-67183, Clone: 1A4E8 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated DMAP1 Monoclonal ant (CL488-67183)

    FC experiment of HeLa using CL488-67183

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human DMAP1 (CL488-67183, Clone:1A4E8) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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CoraLite® Plus 488-conjugated DMAP1 Monoclonal antibody

DMAP1 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-67183
Clone No.1A4E8

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Synonyms

EAF2, DNMTAP1, DNMT1-associated protein 1, DNMT1 associated protein 1, DNMAP1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHEK-293 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67183 targets DMAP1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen DMAP1 fusion protein Ag28906 相同性解析による交差性が予測される生物種
Full Name DNA methyltransferase 1 associated protein 1
Calculated molecular weight 53 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC002855
Gene Symbol DMAP1
Gene ID (NCBI) 55929
RRIDAB_2934526
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9NPF5
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DMAP1 (DNA methyltransferase 1-associated protein 1) a component of the NuA4 histone acetyltransferase complex, was originally identified as an interacting molecule with DNA methyltransferase 1 (DNMT1) which co-localizes with PCNA at DNA replication foci. DMAP1 is required for DNA repair and acts against genomic instability. DMAP1 is widely implicated in transcription repression and activation via its interaction with multiple targets including histone deacetylase2 (HDAC2).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 DMAP1 antibody CL488-67183Download protocol
Standard Protocols
Click here to view our Standard Protocols