DMC1 Monoclonal antibody
DMC1 Monoclonal Antibody for WB, IHC, IF-P, IP, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
Human, mouse, rat
Applications
WB, IHC, IF-P, IP, ELISA
Conjugate
Unconjugated
CloneNo.
2D5C9
Cat no : 67176-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, Jurkat cells, human testis tissue, rat testis, mouse testis |
Positive IP detected in | mouse testis tissue |
Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | mouse testis tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
IF | See 1 publications below |
Product Information
67176-1-Ig targets DMC1 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with Human, mouse, rat samples.
Tested Reactivity | Human, mouse, rat |
Cited Reactivity | mouse |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | DMC1 fusion protein Ag6951 相同性解析による交差性が予測される生物種 |
Full Name | DMC1 dosage suppressor of mck1 homolog, meiosis-specific homologous recombination (yeast) |
Calculated molecular weight | 165 aa, 18 kDa, 38 kDa |
Observed molecular weight | 37 kDa |
GenBank accession number | BC035658 |
Gene symbol | DMC1 |
Gene ID (NCBI) | 11144 |
RRID | AB_2882472 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
Product Specific Protocols | |
---|---|
WB protocol for DMC1 antibody 67176-1-Ig | Download protocol |
IHC protocol for DMC1 antibody 67176-1-Ig | Download protocol |
IF protocol for DMC1 antibody 67176-1-Ig | Download protocol |
IP protocol for DMC1 antibody 67176-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Genet Genomics The novel male meiosis recombination regulator coordinates the progression of meiosis prophase I. |