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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DMC1 Monoclonal antibody

DMC1 Monoclonal Antibody for WB, IHC, IF-P, IP, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IP, ELISA

Conjugate

Unconjugated

CloneNo.

2D5C9

Cat no : 67176-1-Ig

Synonyms

LIM15, HsLim15, DMC1H, 2D5C9

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using DMC1 Monoclonal antibody (67176-1-Ig)

    WB analysis of HeLa using 67176-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 67176-1-Ig (DMC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using DMC1 Monoclonal antibody (67176-1-Ig)

    WB analysis of Jurkat using 67176-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67176-1-Ig (DMC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using DMC1 Monoclonal antibody (67176-1-Ig)

    WB analysis using 67176-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67176-1-Ig (DMC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using DMC1 Monoclonal antibody (67176-1-Ig)

    IP experiment of mouse testis using 67176-1-Ig

    IP result of anti-DMC1 (IP:67176-1-Ig, 4ug; Detection:67176-1-Ig 1:1000) with mouse testis tissue lysate 2040 ug.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using DMC1 Monoclonal antibody (67176-1-Ig)

    IHC staining of mouse testis using 67176-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67176-1-Ig (DMC1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using DMC1 Monoclonal antibody (67176-1-Ig)

    IHC staining of mouse testis using 67176-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67176-1-Ig (DMC1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using DMC1 Monoclonal antibody (67176-1-Ig)

    IF Staining of mouse testis using 67176-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using DMC1 antibody (67176-1-Ig, Clone: 2D5C9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). DAPI (blue).

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using DMC1 Monoclonal antibody (67176-1-Ig)

    IF Staining of mouse testis using 67176-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using DMC1 antibody (67176-1-Ig, Clone: 2D5C9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). DAPI (blue).

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Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, human testis tissue, rat testis, mouse testis
Positive IP detected inmouse testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

67176-1-Ig targets DMC1 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen DMC1 fusion protein Ag6951 相同性解析による交差性が予測される生物種
Full Name DMC1 dosage suppressor of mck1 homolog, meiosis-specific homologous recombination (yeast)
Calculated molecular weight 165 aa, 18 kDa, 38 kDa
Observed molecular weight 37 kDa
GenBank accession numberBC035658
Gene symbol DMC1
Gene ID (NCBI) 11144
RRIDAB_2882472
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for DMC1 antibody 67176-1-IgDownload protocol
IHC protocol for DMC1 antibody 67176-1-IgDownload protocol
IF protocol for DMC1 antibody 67176-1-IgDownload protocol
IP protocol for DMC1 antibody 67176-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

J Genet Genomics

The novel male meiosis recombination regulator coordinates the progression of meiosis prophase I.

Authors - Miao Li
View Article