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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using DMC1 Monoclonal antibody (67176-1-Ig)

    WB analysis of HeLa using 67176-1-Ig (same clone as 67176-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 67176-1-Ig (DMC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67176-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using DMC1 Monoclonal antibody (67176-1-Ig)

    WB analysis of Jurkat using 67176-1-Ig (same clone as 67176-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67176-1-Ig (DMC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67176-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using DMC1 Monoclonal antibody (67176-1-Ig)

    WB analysis using 67176-1-Ig (same clone as 67176-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67176-1-Ig (DMC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67176-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using DMC1 Monoclonal antibody (67176-1-Ig)

    IP experiment of mouse testis using 67176-1-Ig (same clone as 67176-1-PBS)

    IP result of anti-DMC1 (IP:67176-1-Ig, 4ug; Detection:67176-1-Ig 1:1000) with mouse testis tissue lysate 2040 ug. This data was developed using the same antibody clone with 67176-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using DMC1 Monoclonal antibody (67176-1-Ig)

    IHC staining of mouse testis using 67176-1-Ig (same clone as 67176-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67176-1-Ig (DMC1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67176-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using DMC1 Monoclonal antibody (67176-1-Ig)

    IHC staining of mouse testis using 67176-1-Ig (same clone as 67176-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67176-1-Ig (DMC1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67176-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using DMC1 Monoclonal antibody (67176-1-Ig)

    IF Staining of mouse testis using 67176-1-Ig (same clone as 67176-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using DMC1 antibody (67176-1-Ig, Clone: 2D5C9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). DAPI (blue). This data was developed using the same antibody clone with 67176-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using DMC1 Monoclonal antibody (67176-1-Ig)

    IF Staining of mouse testis using 67176-1-Ig (same clone as 67176-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using DMC1 antibody (67176-1-Ig, Clone: 2D5C9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). DAPI (blue). This data was developed using the same antibody clone with 67176-1-PBS in a different storage buffer formulation.

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DMC1 Monoclonal antibody, PBS Only

DMC1 Monoclonal Antibody for WB, IHC, IF-P, IP, Indirect ELISA
Cat No. 67176-1-PBS
Clone No.2D5C9

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IP, Indirect ELISA

Synonyms

LIM15, HsLim15, DMC1H, 2D5C9

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration:  100 μg, 1mg/ml
100 μg, 1mg/ml

¥167,000/ 100 μg

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67176-1-PBS targets DMC1 in WB, IHC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen DMC1 fusion protein Ag6951 相同性解析による交差性が予測される生物種
Full Name DMC1 dosage suppressor of mck1 homolog, meiosis-specific homologous recombination (yeast)
Calculated molecular weight 165 aa, 18 kDa, 38 kDa
Observed molecular weight 37 kDa
GenBank accession numberBC035658
Gene Symbol DMC1
Gene ID (NCBI) 11144
RRIDAB_2882472
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ14565
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.