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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DMC1 Monoclonal antibody, PBS Only

DMC1 Monoclonal Antibody for WB, IHC, IF-P, IP, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D5C9

Cat no : 67176-1-PBS

Synonyms

LIM15, HsLim15, DMC1H, 2D5C9

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using DMC1 Monoclonal antibody (67176-1-Ig)

    WB analysis of HeLa using 67176-1-Ig (same clone as 67176-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 67176-1-Ig (DMC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67176-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using DMC1 Monoclonal antibody (67176-1-Ig)

    WB analysis of Jurkat using 67176-1-Ig (same clone as 67176-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67176-1-Ig (DMC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67176-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using DMC1 Monoclonal antibody (67176-1-Ig)

    WB analysis using 67176-1-Ig (same clone as 67176-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67176-1-Ig (DMC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67176-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using DMC1 Monoclonal antibody (67176-1-Ig)

    IP experiment of mouse testis using 67176-1-Ig (same clone as 67176-1-PBS)

    IP result of anti-DMC1 (IP:67176-1-Ig, 4ug; Detection:67176-1-Ig 1:1000) with mouse testis tissue lysate 2040 ug. This data was developed using the same antibody clone with 67176-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using DMC1 Monoclonal antibody (67176-1-Ig)

    IHC staining of mouse testis using 67176-1-Ig (same clone as 67176-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67176-1-Ig (DMC1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67176-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using DMC1 Monoclonal antibody (67176-1-Ig)

    IHC staining of mouse testis using 67176-1-Ig (same clone as 67176-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67176-1-Ig (DMC1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67176-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using DMC1 Monoclonal antibody (67176-1-Ig)

    IF Staining of mouse testis using 67176-1-Ig (same clone as 67176-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using DMC1 antibody (67176-1-Ig, Clone: 2D5C9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). DAPI (blue). This data was developed using the same antibody clone with 67176-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using DMC1 Monoclonal antibody (67176-1-Ig)

    IF Staining of mouse testis using 67176-1-Ig (same clone as 67176-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using DMC1 antibody (67176-1-Ig, Clone: 2D5C9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). DAPI (blue). This data was developed using the same antibody clone with 67176-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67176-1-PBS targets DMC1 in WB, IHC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen DMC1 fusion protein Ag6951 相同性解析による交差性が予測される生物種
Full Name DMC1 dosage suppressor of mck1 homolog, meiosis-specific homologous recombination (yeast)
Calculated molecular weight 165 aa, 18 kDa, 38 kDa
Observed molecular weight 37 kDa
GenBank accession numberBC035658
Gene symbol DMC1
Gene ID (NCBI) 11144
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.