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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DNAH6 Polyclonal antibody (30073-1-AP)

    WB analysis using 30073-1-AP (same clone as 30073-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 30073-1-AP (DNAH6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30073-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using DNAH6 Polyclonal antibody (30073-1-AP)

    IHC staining of mouse testis using 30073-1-AP (same clone as 30073-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30073-1-AP (DNAH6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30073-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using DNAH6 Polyclonal antibody (30073-1-AP)

    IHC staining of mouse testis using 30073-1-AP (same clone as 30073-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30073-1-AP (DNAH6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30073-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using DNAH6 Polyclonal antibody (30073-1-AP)

    IF Staining of hTERT-RPE1 using 30073-1-AP (same clone as 30073-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using DNAH6 antibody (30073-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), acetylated Tubulin(Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red). This data was developed using the same antibody clone with 30073-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using DNAH6 Polyclonal antibody (30073-1-AP)

    IF Staining of hTERT-RPE1 using 30073-1-AP (same clone as 30073-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using DNAH6 antibody (30073-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite® Plus 488 ARL13B antibody (CL488-17711, green). This data was developed using the same antibody clone with 30073-1-PBS in a different storage buffer formulation.

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DNAH6 Polyclonal antibody, PBS Only

DNAH6 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 30073-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

30073-1-PBS targets DNAH6 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DNAH6 fusion protein Ag32364 相同性解析による交差性が予測される生物種
Full Name dynein, axonemal, heavy chain 6
Calculated molecular weight475 kDa
Observed molecular weight 475 kDa
GenBank accession numberBC104884
Gene Symbol DNAH6
Gene ID (NCBI) 1768
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9C0G6
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

DNAH6(Dynein heavy chain 6, axonemal) is also named as DNAHC6, DNHL1, HL2, KIAA1697 and belongs to the dynein heavy chain family.Dyneins are microtubule-associated motor protein complexes composed of several heavy, light, and intermediate chains(PMID:8812413). It is a force generating protein of respiratory cilia and produces force towards the minus ends of microtubules.This antibody is specific to DNAH6.