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  • Featured Product
  • KD/KO Validated

DNAJB1 Polyclonal antibody

DNAJB1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 13174-1-AP

Synonyms

Heat shock 40 kDa protein 1, hDj-1, Hdj1, hDj 1, DNAJ1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DNAJB1 Polyclonal antibody (13174-1-AP)

    WB analysis using 13174-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13174-1-AP (DNAJB1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using DNAJB1 Polyclonal antibody (13174-1-AP)

    WB analysis using 13174-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13174-1-AP (DNAJB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse lung tissue using DNAJB1 Polyclonal antibody (13174-1-AP)

    IP experiment of mouse lung using 13174-1-AP

    IP result of anti-DNAJB1 (IP:13174-1-AP, 4ug; Detection:13174-1-AP 1:500) with mouse lung tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using DNAJB1 Polyclonal antibody (13174-1-AP)

    IHC staining of mouse testis using 13174-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13174-1-AP (DNAJB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using DNAJB1 Polyclonal antibody (13174-1-AP)

    IHC staining of human oesophagus cancer using 13174-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 13174-1-AP (DNAJB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using DNAJB1 Polyclonal antibody (13174-1-AP)

    IHC staining of human oesophagus cancer using 13174-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 13174-1-AP (DNAJB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using DNAJB1 Polyclonal antibody (13174-1-AP)

    IHC staining of human lymphoma using 13174-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13174-1-AP (DNAJB1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using DNAJB1 Polyclonal antibody (13174-1-AP)

    IHC staining of human lymphoma using 13174-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13174-1-AP (DNAJB1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using DNAJB1 Polyclonal antibody (13174-1-AP)

    IHC staining of mouse testis using 13174-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13174-1-AP (DNAJB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using DNAJB1 Polyclonal antibody (13174-1-AP)

    IF Staining of HeLa using 13174-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DNAJB1 antibody (13174-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using DNAJB1 Polyclonal antibody (13174-1-AP)

    FC experiment of HeLa using 13174-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human DNAJB1 (13174-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive WB detected inHeLa cells, mouse liver tissue, mouse lung tissue, rat lung tissue, rat liver tissue, C2C12 cells, C6 cells
Positive IP detected inmouse lung tissue
Positive IHC detected inhuman oesophagus cancer tissue, human lymphoma tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13174-1-AP targets DNAJB1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DNAJB1 fusion protein Ag3830 相同性解析による交差性が予測される生物種
Full Name DnaJ (Hsp40) homolog, subfamily B, member 1
Calculated molecular weight 340 aa, 40 kDa
Observed molecular weight 37-40 kDa
GenBank accession numberBC019827
Gene symbol DNAJB1
Gene ID (NCBI) 3337
RRIDAB_2230742
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DNAJB1 is a member of the heat shock 40 protein family and has been involved in a variety of cellular processes, including the proteasome pathway, endoplasmic reticulum stress and viral infection. DNAJB1-PRKACA gene fusions have been considered diagnostic for fibrolamellar hepatocellular carcinoma.

Protocols

Product Specific Protocols
WB protocol for DNAJB1 antibody 13174-1-APDownload protocol
IHC protocol for DNAJB1 antibody 13174-1-APDownload protocol
IF protocol for DNAJB1 antibody 13174-1-APDownload protocol
IP protocol for DNAJB1 antibody 13174-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratIF

Dev Cell

Nuclear import carrier Hikeshi cooperates with HSP70 to promote murine oligodendrocyte differentiation and CNS myelination

Authors - Li Li
View Article
humanWB

Proc Natl Acad Sci U S A

Local translation in nuclear condensate amyloid bodies.

Authors - Phaedra R Theodoridis
View Article
mouseWB

Mol Ther Nucleic Acids

Age-associated changes in microglia and astrocytes ameliorate blood-brain barrier dysfunction

Authors - Jie Pan
View Article
humanWB

Int J Mol Sci

PGC1α Promotes Cisplatin Resistance in Ovarian Cancer by Regulating the HSP70/HK2/VDAC1 Signaling Pathway.

Authors - Yanqing Li
View Article
mouseWB

Front Mol Neurosci

Inhibition of Heat Shock Protein 90 by 17-AAG Reduces Inflammation via P2X7 Receptor/NLRP3 Inflammasome Pathway and Increases Neurogenesis After Subarachnoid Hemorrhage in Mice.

Authors - Yuchun Zuo
View Article
mouseWB

J Gerontol A Biol Sci Med Sci

Multifactorial Attenuation of the Murine Heat Shock Response With Age.

Authors - Donald A Jurivich
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Ana (Verified Customer) (03-11-2021)

DNJB1 in mouse samples. 10 ug of total protein. 1:1000 O/N 4ºC incubation in BSA 3% PBST. Secondary ab HRP conjugated 1:5000 1h RT. 2 bands observed.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse Liver & Heart
DNAJB1 Antibody Western Blot validation (1:1000 dilution) in Mouse Liver & Heart (Cat no:13174-1-AP)