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DNAJB1 Polyclonal antibody
DNAJB1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Conjugate
Unconjugated
Cat no : 13174-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, mouse liver tissue, mouse lung tissue, rat lung tissue, rat liver tissue, C2C12 cells, C6 cells |
Positive IP detected in | mouse lung tissue |
Positive IHC detected in | human oesophagus cancer tissue, human lymphoma tissue, mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 4 publications below |
WB | See 29 publications below |
IF | See 1 publications below |
Product Information
13174-1-AP targets DNAJB1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | DNAJB1 fusion protein Ag3830 相同性解析による交差性が予測される生物種 |
Full Name | DnaJ (Hsp40) homolog, subfamily B, member 1 |
Calculated molecular weight | 340 aa, 40 kDa |
Observed molecular weight | 37-40 kDa |
GenBank accession number | BC019827 |
Gene symbol | DNAJB1 |
Gene ID (NCBI) | 3337 |
RRID | AB_2230742 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
DNAJB1 is a member of the heat shock 40 protein family and has been involved in a variety of cellular processes, including the proteasome pathway, endoplasmic reticulum stress and viral infection. DNAJB1-PRKACA gene fusions have been considered diagnostic for fibrolamellar hepatocellular carcinoma.
Protocols
Product Specific Protocols | |
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WB protocol for DNAJB1 antibody 13174-1-AP | Download protocol |
IHC protocol for DNAJB1 antibody 13174-1-AP | Download protocol |
IF protocol for DNAJB1 antibody 13174-1-AP | Download protocol |
IP protocol for DNAJB1 antibody 13174-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Mol Ther Nucleic Acids Age-associated changes in microglia and astrocytes ameliorate blood-brain barrier dysfunction | ||
Int J Mol Sci PGC1α Promotes Cisplatin Resistance in Ovarian Cancer by Regulating the HSP70/HK2/VDAC1 Signaling Pathway. | ||
Front Mol Neurosci Inhibition of Heat Shock Protein 90 by 17-AAG Reduces Inflammation via P2X7 Receptor/NLRP3 Inflammasome Pathway and Increases Neurogenesis After Subarachnoid Hemorrhage in Mice. | ||
J Gerontol A Biol Sci Med Sci Multifactorial Attenuation of the Murine Heat Shock Response With Age. | ||
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Ana (Verified Customer) (03-11-2021) | DNJB1 in mouse samples. 10 ug of total protein. 1:1000 O/N 4ºC incubation in BSA 3% PBST. Secondary ab HRP conjugated 1:5000 1h RT. 2 bands observed.
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