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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 647-conjugated DNAJB1 Polyclonal antibody

DNAJB1 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL647-13174

Synonyms

Heat shock 40 kDa protein 1, hDj-1, Hdj1, hDj 1, DNAJ1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 647-conjugated DNAJB1 Polyclonal an (CL647-13174)

    IF Staining of HeLa using CL647-13174

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 647 DNAJB1 antibody (CL647-13174) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 647-conjugated DNAJB1 Polyclonal an (CL647-13174)

    FC experiment of HeLa using CL647-13174

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human DNAJB1 (CL647-13174) (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-13174 targets DNAJB1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DNAJB1 fusion protein Ag3830 相同性解析による交差性が予測される生物種
Full Name DnaJ (Hsp40) homolog, subfamily B, member 1
Calculated molecular weight 340 aa, 40 kDa
Observed molecular weight 37-40 kDa
GenBank accession numberBC019827
Gene symbol DNAJB1
Gene ID (NCBI) 3337
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DNAJB1 is a member of the heat shock 40 protein family and has been involved in a variety of cellular processes, including the proteasome pathway, endoplasmic reticulum stress and viral infection. DNAJB1-PRKACA gene fusions have been considered diagnostic for fibrolamellar hepatocellular carcinoma.

Protocols

Product Specific Protocols
IF protocol for CL Plus 647 DNAJB1 antibody CL647-13174Download protocol
FC protocol for CL Plus 647 DNAJB1 antibody CL647-13174Download protocol
Standard Protocols
Click here to view our Standard Protocols