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  • KD/KO Validated

DNAJB4 Polyclonal antibody

DNAJB4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 13064-1-AP

Synonyms

Human liver DnaJ like protein, HSP40 homolog, HLJ1, Heat shock protein 40 homolog, DNAJW


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Tested Applications

Positive WB detected inHeLa cells, mouse serum, mouse skeletal muscle, rat skeletal muscle
Positive IP detected inHeLa cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13064-1-AP targets DNAJB4 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DNAJB4 fusion protein Ag3756 相同性解析による交差性が予測される生物種
Full Name DnaJ (Hsp40) homolog, subfamily B, member 4
Calculated molecular weight 38 kDa
Observed molecular weight 38 kDa
GenBank accession numberBC034721
Gene symbol DNAJB4
Gene ID (NCBI) 11080
RRIDAB_2094422
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for DNAJB4 antibody 13064-1-APDownload protocol
IHC protocol for DNAJB4 antibody 13064-1-APDownload protocol
IF protocol for DNAJB4 antibody 13064-1-APDownload protocol
IP protocol for DNAJB4 antibody 13064-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IF

Acta Neuropathol

Distinctive chaperonopathy in skeletal muscle associated with the dominant variant in DNAJB4

Authors - Michio Inoue
mouseWB

Int J Mol Sci

Genetic Deletion of HLJ1 Does Not Affect Blood Coagulation in Mice.

Authors - Man-Chen Hsu
  • KO Validated
humanWB

OMICS

HLJ1 (DNAJB4) Gene Is a Novel Biomarker Candidate in Breast Cancer.

Authors - Tolga Acun
humanWB,IHC

Int J Clin Exp Pathol

HLJ1 is a novel biomarker for colorectal carcinoma progression and overall patient survival.

Authors - Yong Liu
mouseWB

Mol Ther Nucleic Acids

DNAJB6 isoform specific knockdown: Therapeutic potential for limb girdle muscular dystrophy D1

Authors - Andrew R Findlay
humanIHC,WB

Discov Oncol

DNAJB4 suppresses breast cancer progression and promotes tumor immunity by regulating the Hippo signaling pathway

Authors - Yanru Chen