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KD/KO Validated

DNAJB4 Polyclonal antibody

Name: DNAJB4 Polyclonal antibody
Brand: Proteintech
SKU: 13064-1-AP

DNAJB4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 13064-1-AP

Synonyms

Human liver DnaJ like protein, HSP40 homolog, HLJ1, Heat shock protein 40 homolog, DNAJW

Validation Data Gallery

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AllWBIPIHCIF/ICC

WB analysis using 13064-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13064-1-AP (DNAJB4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis using 13064-1-AP
Mouse serum lysates were subjected to SDS PAGE followed by western blot with 13064-1-AP (DNAJB4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 13064-1-AP
IP result of anti-DNAJB4 (IP:13064-1-AP, 4ug; Detection:13064-1-AP 1:2000) with HeLa cells lysate 2600 ug.
IHC staining of human pancreas cancer using 13064-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 13064-1-AP (DNAJB4 antibody) at dilution of 1:100 (under 40x lens).
IF Staining of MCF-7 using 13064-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using DNAJB4 antibody (13064-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 13064-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, mouse serum, mouse skeletal muscle, rat skeletal muscle
Positive IP detected in	HeLa cells
Positive IHC detected in	human pancreas cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 4 publications below
WB	See 10 publications below
IHC	See 2 publications below
IF	See 2 publications below
IP	See 1 publications below

Product Information

13064-1-AP targets DNAJB4 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	DNAJB4 fusion protein Ag3756 相同性解析による交差性が予測される生物種
Full Name	DnaJ (Hsp40) homolog, subfamily B, member 4
Calculated molecular weight	38 kDa
Observed molecular weight	38 kDa
GenBank accession number	BC034721
Gene symbol	DNAJB4
Gene ID (NCBI)	11080
RRID	AB_2094422
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for DNAJB4 antibody 13064-1-AP	Download protocol
IHC protocol for DNAJB4 antibody 13064-1-AP	Download protocol
IF protocol for DNAJB4 antibody 13064-1-AP	Download protocol
IP protocol for DNAJB4 antibody 13064-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human,mouse	WB,IF	Acta Neuropathol Distinctive chaperonopathy in skeletal muscle associated with the dominant variant in DNAJB4 Authors - Michio Inoue View Article
mouse	WB	Int J Mol Sci Genetic Deletion of HLJ1 Does Not Affect Blood Coagulation in Mice. Authors - Man-Chen Hsu KO Validated View Article
human	WB	OMICS HLJ1 (DNAJB4) Gene Is a Novel Biomarker Candidate in Breast Cancer. Authors - Tolga Acun View Article
human	WB,IHC	Int J Clin Exp Pathol HLJ1 is a novel biomarker for colorectal carcinoma progression and overall patient survival. Authors - Yong Liu View Article
mouse	WB	Elife HLJ1 amplifies endotoxin-induced sepsis severity by promoting IL-12 heterodimerization in macrophages Authors - Wei-Jia Luo KD Validated View Article
mouse	WB	Acta Neuropathol Loss of function variants in DNAJB4 cause a myopathy with early respiratory failure Authors - Conrad C Weihl KO Validated View Article

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研究分野

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DNAJB4 Polyclonal antibody

DNAJB4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis using 13064-1-AP

WB analysis using 13064-1-AP

IP experiment of HeLa using 13064-1-AP

IHC staining of human pancreas cancer using 13064-1-AP

IF Staining of MCF-7 using 13064-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Distinctive chaperonopathy in skeletal muscle associated with the dominant variant in DNAJB4

Genetic Deletion of HLJ1 Does Not Affect Blood Coagulation in Mice.

HLJ1 (DNAJB4) Gene Is a Novel Biomarker Candidate in Breast Cancer.

HLJ1 is a novel biomarker for colorectal carcinoma progression and overall patient survival.

HLJ1 amplifies endotoxin-induced sepsis severity by promoting IL-12 heterodimerization in macrophages

Loss of function variants in DNAJB4 cause a myopathy with early respiratory failure