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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated DNAJB4 Polyclonal antibody

DNAJB4 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-13064

Synonyms

Human liver DnaJ like protein, HSP40 homolog, HLJ1, Heat shock protein 40 homolog, DNAJW

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CoraLite® Plus 488-conjugated DNAJB4 Polyclonal an (CL488-13064)

    IF Staining of MCF-7 using CL488-13064

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite® Plus 488 DNAJB4 antibody (CL488-13064) at dilution of 1:200.

  • Flow cytometry (FC) experiment of MCF-7 cells using CoraLite® Plus 488-conjugated DNAJB4 Polyclonal an (CL488-13064)

    FC experiment of MCF-7 using CL488-13064

    1x10^6 MCF-7 cells were intracellularly stained with 0.4 ug Coralite® Plus 488 Dnajb4 Polyclonal Antibody (CL488-13064)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-13064 targets DNAJB4 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DNAJB4 fusion protein Ag3756 相同性解析による交差性が予測される生物種
Full Name DnaJ (Hsp40) homolog, subfamily B, member 4
Calculated molecular weight 38 kDa
Observed molecular weight 38 kDa
GenBank accession numberBC034721
Gene symbol DNAJB4
Gene ID (NCBI) 11080
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 DNAJB4 antibody CL488-13064Download protocol
FC protocol for CL Plus 488 DNAJB4 antibody CL488-13064Download protocol
Standard Protocols
Click here to view our Standard Protocols