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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DNAJB4 Monoclonal antibody

DNAJB4 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig, rabbit, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1F3H7

Cat no : 67966-1-Ig

Synonyms

DjB4, DNAJB4, DNAJW, Heat shock protein 40 homolog, HLJ1, HSP40 homolog, Human liver DnaJ like protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DNAJB4 Monoclonal antibody (67966-1-Ig)

    WB analysis using 67966-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67966-1-Ig (DNAJB4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using DNAJB4 Monoclonal antibody (67966-1-Ig)

    WB analysis of mouse heart using 67966-1-Ig

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 67966-1-Ig (DNAJB4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using DNAJB4 Monoclonal antibody (67966-1-Ig)

    IHC staining of human pancreas cancer using 67966-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67966-1-Ig (DNAJB4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using DNAJB4 Monoclonal antibody (67966-1-Ig)

    IHC staining of human pancreas cancer using 67966-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67966-1-Ig (DNAJB4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using DNAJB4 Monoclonal antibody (67966-1-Ig)

    IF Staining of A431 using 67966-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using DNAJB4 antibody (67966-1-Ig, Clone: 1F3H7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inA549 cells, mouse heart tissue, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, pig heart, rabbit heart
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67966-1-Ig targets DNAJB4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, pig, rabbit, mouse samples.

Tested Reactivity Human, pig, rabbit, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen DNAJB4 fusion protein Ag3756 相同性解析による交差性が予測される生物種
Full Name DnaJ (Hsp40) homolog, subfamily B, member 4
Calculated molecular weight 38 kDa
Observed molecular weight38 kDa
GenBank accession numberBC034721
Gene symbol DNAJB4
Gene ID (NCBI) 11080
RRIDAB_2918717
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for DNAJB4 antibody 67966-1-IgDownload protocol
IHC protocol for DNAJB4 antibody 67966-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols