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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DNAJB4 Monoclonal antibody (67966-1-Ig)

    WB analysis using 67966-1-Ig (same clone as 67966-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67966-1-Ig (DNAJB4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67966-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse heart tissue using DNAJB4 Monoclonal antibody (67966-1-Ig)

    WB analysis of mouse heart using 67966-1-Ig (same clone as 67966-1-PBS)

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 67966-1-Ig (DNAJB4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67966-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using DNAJB4 Monoclonal antibody (67966-1-Ig)

    IHC staining of human pancreas cancer using 67966-1-Ig (same clone as 67966-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67966-1-Ig (DNAJB4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67966-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using DNAJB4 Monoclonal antibody (67966-1-Ig)

    IHC staining of human pancreas cancer using 67966-1-Ig (same clone as 67966-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67966-1-Ig (DNAJB4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67966-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using DNAJB4 Monoclonal antibody (67966-1-Ig)

    IF Staining of A431 using 67966-1-Ig (same clone as 67966-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using DNAJB4 antibody (67966-1-Ig, Clone: 1F3H7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67966-1-PBS in a different storage buffer formulation.

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DNAJB4 Monoclonal antibody, PBS Only

DNAJB4 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67966-1-PBS
Clone No.1F3H7

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig, rabbit, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

DjB4, DNAJB4, DNAJW, Heat shock protein 40 homolog, HLJ1, HSP40 homolog, Human liver DnaJ like protein

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67966-1-PBS targets DNAJB4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, pig, rabbit, mouse samples.

Tested Reactivity Human, pig, rabbit, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen DNAJB4 fusion protein Ag3756 相同性解析による交差性が予測される生物種
Full Name DnaJ (Hsp40) homolog, subfamily B, member 4
Calculated molecular weight 38 kDa
Observed molecular weight38 kDa
GenBank accession numberBC034721
Gene Symbol DNAJB4
Gene ID (NCBI) 11080
RRIDAB_2918717
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9UDY4
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.