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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DNMT1 Recombinant antibody, PBS Only (Detector)

DNMT1 Recombinant Antibody for Cytometric bead array, Indirect ELISA, WB

Host / Isotype

Rabbit / IgG

Reactivity

rat, human

Applications

Cytometric bead array, Indirect ELISA, WB

Conjugate

Unconjugated

CloneNo.

240302E2

Cat no : 82678-2-PBS

Synonyms

DNMT, DNA (cytosine-5)-methyltransferase 1, CXXC-type zinc finger protein 9, CXXC9, AIM

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using DNMT1 Recombinant antibody (82678-2-RR)

    WB analysis using 82678-2-RR (same clone as 82678-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82678-2-RR (DNMT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82678-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using DNMT1 Recombinant antibody (82678-2-RR)

    FC experiment of HeLa using 82678-2-RR (same clone as 82678-2-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug DNMT1 Recombinant antibody (82678-2-RR, Clone:240302E2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 82678-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00377-1

    Cytometric bead array standard curve of MP00377-1

    Cytometric bead array standard curve of MP00377-1, DNMT1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82678-3-PBS. Detection antibody: 82678-2-PBS. Standard: Ag29479. Range: 1.56-100 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82678-2-PBS targets DNMT1 as part of a matched antibody pair:

MP00377-1: 82678-3-PBS capture and 82678-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity rat, human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen DNMT1 fusion protein Ag29479 相同性解析による交差性が予測される生物種
Full Name DNA (cytosine-5-)-methyltransferase 1
Calculated molecular weight 1632 aa, 185 kDa
GenBank accession numberBC126227
Gene symbol DNMT1
Gene ID (NCBI) 1786
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.