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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DNMT1 Recombinant antibody

DNMT1 Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240302E2

Cat no : 82678-2-RR

Synonyms

DNMT, DNA (cytosine-5)-methyltransferase 1, CXXC-type zinc finger protein 9, CXXC9, AIM

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DNMT1 Recombinant antibody (82678-2-RR)

    WB analysis using 82678-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82678-2-RR (DNMT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HeLa cells using DNMT1 Recombinant antibody (82678-2-RR)

    FC experiment of HeLa using 82678-2-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug DNMT1 Recombinant antibody (82678-2-RR, Clone:240302E2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive WB detected inHepG2 cells, HEK-293T cells, PC-12 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 82678-2-RR is DNMT1 Fusion Protein expressed in E. coli.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen DNMT1 fusion protein Ag29479 相同性解析による交差性が予測される生物種
Full Name DNA (cytosine-5-)-methyltransferase 1
Calculated molecular weight 1632 aa, 185 kDa
Observed molecular weight200 kDa
GenBank accession numberBC126227
Gene symbol DNMT1
Gene ID (NCBI) 1786
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DNA methylation is a major epigenetic modification that regulates gene expression. DNMT1, the maintenance DNA methylation enzyme, is the primary enzyme responsible for copying methylation patterns after DNA replication. DNMT1 is required for X chromosome inactivation, imprinting, genomic methylation, and proper embryonic development. Overexpression of DNMT1 has been reported in various cancers. (PMID: 10753866)

Protocols

Product Specific Protocols
WB protocol for DNMT1 antibody 82678-2-RRDownload protocol
FC protocol for DNMT1 antibody 82678-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols