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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated DOCK8 Recombinant antibody

DOCK8 Recombinant Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

230441A2

Cat no : CL488-83270-2

Synonyms

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of THP-1 cells using CoraLite® Plus 488-conjugated DOCK8 Recombinant an (CL488-83270-2)

    IF Staining of THP-1 using CL488-83270-2

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CoraLite® Plus 488 DOCK8 antibody (CL488-83270-2, Clone: 230441A2 ) at dilution of 1:200.

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Tested Applications

Positive IF/ICC detected inTHP-1 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-83270-2 targets DOCK8 in IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen DOCK8 fusion protein Ag34585 相同性解析による交差性が予測される生物種
Full Name dedicator of cytokinesis 8
Calculated molecular weight 2031 aa, 231 kDa
Observed molecular weight239 kDa
GenBank accession numberBC019102
Gene symbol DOCK8
Gene ID (NCBI) 81704
RRIDAB_3673231
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DOCK8 is a member of the DOCK family of proteins, which have a unique DRH2 domain enabling them to act as GEFs and so controlling a range of cellular processes in various signaling pathways (PMID: 12432077). The specific target of DOCK8 is Cell division control protein 42 homolog (Cdc42), a small GTPase that is involved in regulation of the cell cycle and forms a complex. DOCK8 also acts as a scaffold molecule in this complex that initiates actin polymerization via the Wiskott-Aldrich Syndrome protein (WASp) (PubMed: 28028151, PubMed: 22461490).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 DOCK8 antibody CL488-83270-2Download protocol
Standard Protocols
Click here to view our Standard Protocols