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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of LNCaP cells using CoraLite® Plus 488-conjugated DOHH Monoclonal anti (CL488-68151)

    IF Staining of LNCaP using CL488-68151

    Immunofluorescent analysis of (-20°C Ethanol) fixed LNCaP cells using CoraLite® Plus 488 DOHH antibody (CL488-68151, Clone: 1H5H7 ) at dilution of 1:200, CL594-Phalloidin (red).

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CoraLite® Plus 488-conjugated DOHH Monoclonal antibody

DOHH Monoclonal Antibody for IF/ICC
Cat No. CL488-68151
Clone No.1H5H7

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC

Synonyms

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inLNCaP cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68151 targets DOHH in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen DOHH fusion protein Ag13041 相同性解析による交差性が予測される生物種
Full Name deoxyhypusine hydroxylase/monooxygenase
Calculated molecular weight 33 kDa
Observed molecular weight30-35 kDa
GenBank accession numberBC002817
Gene Symbol DOHH
Gene ID (NCBI) 83475
RRIDAB_3672975
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9BU89
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Deoxyhypusine hydroxylase (DOHH) is the enzyme catalyzing the second step in the post-translational synthesis of hypusine [Nε-(4-amino-2-hydroxybutyl)lysine] in the eukaryotic initiation factor 5A (eIF5A). DOHH has been reported to mediate several crucial cellular functions, including cellular proliferation, differentiation and apoptosis. Moreover, previous studies have established that DOHH is highly involved in several essential biological processes driving human diseases including cancer growth, malarial drug resistance, and HIV-1 replication. In particular, the DOHH/eIF5A signaling pathway was revealed to mediate the ability of nerve growth factor to enhance neuronal growth and survival, highlighting the importance of DOHH in neuroprotection(PMID: 35007708, PMID: 16371467, PMID: 22908221, PMID: 19706422).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 DOHH antibody CL488-68151Download protocol
Standard Protocols
Click here to view our Standard Protocols