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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DPP4/CD26 Monoclonal antibody

DPP4/CD26 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig, rabbit

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2G6D11

Cat no : 68383-1-Ig

Synonyms

CD26, ADCP-2, ADCP2, ADCP 2, ADABP

Validation Data Gallery

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  • Western Blot (WB) analysis of human placenta tissue using DPP4/CD26 Monoclonal antibody (68383-1-Ig)

    WB analysis of human placenta using 68383-1-Ig

    human placenta tissue were subjected to SDS PAGE followed by western blot with 68383-1-Ig (DPP4/CD26 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HuH-7 cells using DPP4/CD26 Monoclonal antibody (68383-1-Ig)

    WB analysis of HuH-7 using 68383-1-Ig

    HuH-7 cells were subjected to SDS PAGE followed by western blot with 68383-1-Ig (DPP4/CD26 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig kidney tissue using DPP4/CD26 Monoclonal antibody (68383-1-Ig)

    WB analysis of pig kidney using 68383-1-Ig

    pig kidney tissue were subjected to SDS PAGE followed by western blot with 68383-1-Ig (DPP4/CD26 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rabbit kidney tissue using DPP4/CD26 Monoclonal antibody (68383-1-Ig)

    WB analysis of rabbit kidney using 68383-1-Ig

    rabbit kidney tissue were subjected to SDS PAGE followed by western blot with 68383-1-Ig (DPP4/CD26 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HuH-7 cells using DPP4/CD26 Monoclonal antibody (68383-1-Ig)

    IF Staining of HuH-7 using 68383-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HuH-7 cells using DPP4/CD26 antibody (68383-1-Ig, Clone: 2G6D11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inhuman placenta tissue, HuH-7 cells, pig kidney tissue, rabbit kidney tissue
Positive IF/ICC detected inHuH-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

68383-1-Ig targets DPP4/CD26 in WB, IF/ICC, ELISA applications and shows reactivity with human, pig, rabbit samples.

Tested Reactivity human, pig, rabbit
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name dipeptidyl-peptidase 4
Calculated molecular weight 88 kDa
Observed molecular weight110 kDa
GenBank accession numberBC013329
Gene symbol DPP4
Gene ID (NCBI) 1803
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD26, also known as DPP4 (dipeptidyl peptidase-4), is a 110 kDa type II cell-surface glycoprotein widely expressed on T cells, activated B cells, activated NK cells and myeloid cells as well as on epithelial cells, fibroblasts, mesothelium, and endothelial cells of a variety of tissues (PMID:33777580; 26919392). It has ecto-enzyme activity in its extracellular domain and cleaves amino-terminal dipeptides with either L-proline or L-alanine at the penultimate position (PMID: 9553764). CD26 plays roles in diverse biological processes such as immunoregulation, glucose homeostasis, and tumorigenesis (PMID: 33777580).

Protocols

Product Specific Protocols
WB protocol for DPP4/CD26 antibody 68383-1-IgDownload protocol
IF protocol for DPP4/CD26 antibody 68383-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Stem Cell Res Ther

Single-cell sequencing of facial adipose tissue unveils FKBP5 as a therapeutic target for facial infiltrating lipomatosis

Authors - Hongrui Chen
View Article