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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DPP4/CD26 Monoclonal antibody, PBS Only (Capture)

DPP4/CD26 Monoclonal Antibody for WB, IF/ICC, Sandwich ELISA, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig, rabbit

Applications

WB, IF/ICC, Sandwich ELISA, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2G6D11

Cat no : 68383-1-PBS

Synonyms

CD26, ADCP-2, ADCP2, ADCP 2, ADABP

Validation Data Gallery

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  • Western Blot (WB) analysis of human placenta tissue using DPP4/CD26 Monoclonal antibody (68383-1-Ig)

    WB analysis of human placenta using 68383-1-Ig (same clone as 68383-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 68383-1-Ig (DPP4/CD26 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68383-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig kidney tissue using DPP4/CD26 Monoclonal antibody (68383-1-Ig)

    WB analysis of pig kidney using 68383-1-Ig (same clone as 68383-1-PBS)

    pig kidney tissue were subjected to SDS PAGE followed by western blot with 68383-1-Ig (DPP4/CD26 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68383-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rabbit kidney tissue using DPP4/CD26 Monoclonal antibody (68383-1-Ig)

    WB analysis of rabbit kidney using 68383-1-Ig (same clone as 68383-1-PBS)

    rabbit kidney tissue were subjected to SDS PAGE followed by western blot with 68383-1-Ig (DPP4/CD26 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68383-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HuH-7 cells using DPP4/CD26 Monoclonal antibody (68383-1-Ig)

    WB analysis of HuH-7 using 68383-1-Ig (same clone as 68383-1-PBS)

    HuH-7 cells were subjected to SDS PAGE followed by western blot with 68383-1-Ig (DPP4/CD26 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68383-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP50051-1

    Sandwich ELISA standard curve of MP50051-1

    Sandwich ELISA standard curve of MP50051-1, DPP4 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68383-1-PBS. Detection antibody: 68383-2-PBS. Standard: Eg0157. Range: 0.313-20 ng/mL.

  • Immunofluorescence (IF) / fluorescent staining of HuH-7 cells using DPP4/CD26 Monoclonal antibody (68383-1-Ig)

    IF Staining of HuH-7 using 68383-1-Ig (same clone as 68383-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HuH-7 cells using DPP4/CD26 antibody (68383-1-Ig, Clone: 2G6D11 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68383-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68383-1-PBS targets DPP4/CD26 as part of a matched antibody pair:

MP50051-1: 68383-1-PBS capture and 68383-2-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, pig, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name dipeptidyl-peptidase 4
Calculated molecular weight 88 kDa
Observed molecular weight110 kDa
GenBank accession numberBC013329
Gene symbol DPP4
Gene ID (NCBI) 1803
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD26, also known as DPP4 (dipeptidyl peptidase-4), is a 110 kDa type II cell-surface glycoprotein widely expressed on T cells, activated B cells, activated NK cells and myeloid cells as well as on epithelial cells, fibroblasts, mesothelium, and endothelial cells of a variety of tissues (PMID:33777580; 26919392). It has ecto-enzyme activity in its extracellular domain and cleaves amino-terminal dipeptides with either L-proline or L-alanine at the penultimate position (PMID: 9553764). CD26 plays roles in diverse biological processes such as immunoregulation, glucose homeostasis, and tumorigenesis (PMID: 33777580).