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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DPP4/CD26 Monoclonal antibody, PBS Only (Capture)

DPP4/CD26 Monoclonal Antibody for WB, FC, Sandwich ELISA, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Pig, Rabbit

Applications

WB, FC, Sandwich ELISA, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2G6D11

Cat no : 68383-1-PBS

Synonyms

ADABP, ADCP 2, ADCP2, CD26, dipeptidyl peptidase 4, Dipeptidyl peptidase IV, DPP IV, DPP4, DPP4/CD26, DPPIV, T cell activation antigen CD26, TP103

Validation Data Gallery

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  • Western Blot (WB) analysis of human placenta tissue using DPP4/CD26 Monoclonal antibody (68383-1-Ig)

    WB analysis of human placenta using 68383-1-Ig (same clone as 68383-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 68383-1-Ig (DPP4/CD26 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68383-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig kidney tissue using DPP4/CD26 Monoclonal antibody (68383-1-Ig)

    WB analysis of pig kidney using 68383-1-Ig (same clone as 68383-1-PBS)

    pig kidney tissue were subjected to SDS PAGE followed by western blot with 68383-1-Ig (DPP4/CD26 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68383-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rabbit kidney tissue using DPP4/CD26 Monoclonal antibody (68383-1-Ig)

    WB analysis of rabbit kidney using 68383-1-Ig (same clone as 68383-1-PBS)

    rabbit kidney tissue were subjected to SDS PAGE followed by western blot with 68383-1-Ig (DPP4/CD26 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68383-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HuH-7 cells using DPP4/CD26 Monoclonal antibody (68383-1-Ig)

    WB analysis of HuH-7 using 68383-1-Ig (same clone as 68383-1-PBS)

    HuH-7 cells were subjected to SDS PAGE followed by western blot with 68383-1-Ig (DPP4/CD26 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68383-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP50051-1

    Sandwich ELISA standard curve of MP50051-1

    Sandwich ELISA standard curve of MP50051-1, DPP4 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68383-1-PBS. Detection antibody: 68383-2-PBS. Standard: Eg0157. Range: 0.313-20 ng/mL.

  • Flow cytometry (FC) experiment of HuH-7 cells using DPP4/CD26 Monoclonal antibody (68383-1-Ig)

    FC experiment of HuH-7 using 68383-1-Ig (same clone as 68383-1-PBS)

    1X10^6 HuH-7 cells were surface stained with 0.4 ug Anti-Human DPP4/CD26 (68383-1-Ig, Clone:2G6D11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were not fixed . This data was developed using the same antibody clone with 68383-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68383-1-PBS targets DPP4/CD26 as part of a matched antibody pair:

MP50051-1: 68383-1-PBS capture and 68383-2-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity Human, Pig, Rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name dipeptidyl-peptidase 4
Calculated molecular weight 88 kDa
Observed molecular weight110 kDa
GenBank accession numberBC013329
Gene symbol DPP4
Gene ID (NCBI) 1803
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

DPP4 (also known as CD26) is a serine exopeptidase that cleaves X-proline dipeptides from the N terminus of polypeptides. It is an intrinsic membrane glycoprotein anchored into the cell membrane by its N-terminal end. High levels of the enzyme are found in the brush-border membranes of the kidney proximal tubule and of the small intestine, but several other tissues also express the enzyme. The enzyme is present in the fetal colon but disappears at birth. It is ectopically expressed in some human colon adenocarcinomas and human colon cancer cell lines (PMID:1977364).