• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DR6 Monoclonal antibody, PBS Only

DR6 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3C2D10

Cat no : 66754-1-PBS

Synonyms

BM 018, CD358, Death receptor 6, DR6, TNFRSF21

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of K-562 cells using DR6 Monoclonal antibody (66754-1-Ig)

    WB analysis of K-562 using 66754-1-Ig (same clone as 66754-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 66754-1-Ig (DR6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66754-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using DR6 Monoclonal antibody (66754-1-Ig)

    WB analysis of Jurkat using 66754-1-Ig (same clone as 66754-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66754-1-Ig (DR6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66754-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Raji cells using DR6 Monoclonal antibody (66754-1-Ig)

    WB analysis of Raji using 66754-1-Ig (same clone as 66754-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 66754-1-Ig (DR6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66754-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Daudi cells using DR6 Monoclonal antibody (66754-1-Ig)

    WB analysis of Daudi using 66754-1-Ig (same clone as 66754-1-PBS)

    Daudi cells were subjected to SDS PAGE followed by western blot with 66754-1-Ig (DR6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66754-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of THP-1 cells using DR6 Monoclonal antibody (66754-1-Ig)

    WB analysis of THP-1 using 66754-1-Ig (same clone as 66754-1-PBS)

    THP-1 cells were subjected to SDS PAGE followed by western blot with 66754-1-Ig (DR6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66754-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using DR6 Monoclonal antibody (66754-1-Ig)

    IF Staining of HepG2 using 66754-1-Ig (same clone as 66754-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DR6 antibody (66754-1-Ig, Clone: 3C2D10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66754-1-PBS in a different storage buffer formulation.

☆大好評トライアルサイズ ¥20,000～！ >>> 一次抗体特別価格キャンペーン開催中！

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66754-1-PBS targets DR6 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen DR6 fusion protein Ag14327 相同性解析による交差性が予測される生物種
Full Name tumor necrosis factor receptor superfamily, member 21
Calculated molecular weight 655 aa, 72 kDa
Observed molecular weight 69 kDa
GenBank accession numberBC021572
Gene symbol DR6
Gene ID (NCBI) 27242
RRIDAB_2882100
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

DR6 (also known as TNFRSF21 or CD358) is an orphan receptor of the tumor necrosis factor (TNF) receptor superfamily. It controls cell death and differentiation in a cell-autonomous manner in different cell types including neurons, oligodendrocytes, endothelial cells, and immune cells (PMID: 29459438). DR6 has been shown to activate NF-κB and JNK, and induce cell apoptosis (PMID: 9714541). DR6 interacts with TRADD, which is known to mediate signal transduction of TNF receptors. It has been reported that APP binds DR6 to trigger axon pruning and neuron death via distinct caspases, indicating that DR6 might be involved in Alzheimer's disease (PMID: 19225519).