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Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using TNFRSF21 Monoclonal antibody (66754-1-Ig)

    WB analysis of K-562 using 66754-1-Ig (same clone as 66754-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 66754-1-Ig (DR6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66754-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using TNFRSF21 Monoclonal antibody (66754-1-Ig)

    WB analysis of Jurkat using 66754-1-Ig (same clone as 66754-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66754-1-Ig (DR6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66754-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Raji cells using TNFRSF21 Monoclonal antibody (66754-1-Ig)

    WB analysis of Raji using 66754-1-Ig (same clone as 66754-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 66754-1-Ig (DR6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66754-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Daudi cells using TNFRSF21 Monoclonal antibody (66754-1-Ig)

    WB analysis of Daudi using 66754-1-Ig (same clone as 66754-1-PBS)

    Daudi cells were subjected to SDS PAGE followed by western blot with 66754-1-Ig (DR6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66754-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of THP-1 cells using TNFRSF21 Monoclonal antibody (66754-1-Ig)

    WB analysis of THP-1 using 66754-1-Ig (same clone as 66754-1-PBS)

    THP-1 cells were subjected to SDS PAGE followed by western blot with 66754-1-Ig (DR6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66754-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TNFRSF21 Monoclonal antibody (66754-1-Ig)

    IF Staining of HepG2 using 66754-1-Ig (same clone as 66754-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DR6 antibody (66754-1-Ig, Clone: 3C2D10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66754-1-PBS in a different storage buffer formulation.

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TNFRSF21 Monoclonal antibody, PBS Only

TNFRSF21 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 66754-1-PBS
Clone No.3C2D10

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

DR6, UNQ437/PRO868, Death receptor 6, CD358, 3C2D10

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66754-1-PBS targets TNFRSF21 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TNFRSF21 fusion protein Ag14327 相同性解析による交差性が予測される生物種
Full Name tumor necrosis factor receptor superfamily, member 21
Calculated molecular weight 655 aa, 72 kDa
Observed molecular weight 69 kDa
GenBank accession numberBC021572
Gene Symbol DR6
Gene ID (NCBI) 27242
RRIDAB_2882100
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO75509
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

TNFRSF21 (also known as DR6 or CD358) is an orphan receptor of the tumor necrosis factor (TNF) receptor superfamily. It controls cell death and differentiation in a cell-autonomous manner in different cell types including neurons, oligodendrocytes, endothelial cells, and immune cells (PMID: 29459438). DR6 has been shown to activate NF-κB and JNK, and induce cell apoptosis (PMID: 9714541). DR6 interacts with TRADD, which is known to mediate signal transduction of TNF receptors. It has been reported that APP binds DR6 to trigger axon pruning and neuron death via distinct caspases, indicating that DR6 might be involved in Alzheimer's disease (PMID: 19225519).