Validation Data Gallery
Tested Applications
| Positive WB detected in | K-562 cells, THP-1 cells, Jurkat cells, Raji cells, Daudi cells |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
66754-1-Ig targets TNFRSF21 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | TNFRSF21 fusion protein Ag14327 相同性解析による交差性が予測される生物種 |
| Full Name | tumor necrosis factor receptor superfamily, member 21 |
| Calculated molecular weight | 655 aa, 72 kDa |
| Observed molecular weight | 69 kDa |
| GenBank accession number | BC021572 |
| Gene Symbol | DR6 |
| Gene ID (NCBI) | 27242 |
| RRID | AB_2882100 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | O75509 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TNFRSF21 (also known as DR6 or CD358) is an orphan receptor of the tumor necrosis factor (TNF) receptor superfamily. It controls cell death and differentiation in a cell-autonomous manner in different cell types including neurons, oligodendrocytes, endothelial cells, and immune cells (PMID: 29459438). DR6 has been shown to activate NF-κB and JNK, and induce cell apoptosis (PMID: 9714541). DR6 interacts with TRADD, which is known to mediate signal transduction of TNF receptors. It has been reported that APP binds DR6 to trigger axon pruning and neuron death via distinct caspases, indicating that DR6 might be involved in Alzheimer's disease (PMID: 19225519).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TNFRSF21 antibody 66754-1-Ig | Download protocol |
| IF protocol for TNFRSF21 antibody 66754-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Death Discov Knockdown of KIF15 suppresses proliferation of prostate cancer cells and induces apoptosis through PI3K/Akt signaling pathway |